| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,602,232 | T→A | 12.1% | D434V (GAT→GTT) | PP_4924 ← | subtilase family serine protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,602,232 | 0 | T | A | 12.1% | 91.7 / 5.4 | 33 | D434V (GAT→GTT) | PP_4924 | subtilase family serine protease |
| Reads supporting (aligned to +/- strand): ref base T (13/16); new base A (2/2); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CTACCGGCTCGACCTGGCCGAGCTTGATCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAGCTGGCTGACGTTGCTCACCTGGCCGGTACCGTTGTC > NC_002947/5602083‑5602374 | cTACCGGCTCGACCTGGCCGAGCTTGATCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCAt > 1:185193/1‑150 (MQ=255) tCGACCTGGCCGAGCTTGATCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTg > 1:253656/1‑150 (MQ=255) aCCTGGCCGAGCTTGATCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCg > 2:486491/1‑150 (MQ=255) cGAGCTTGATCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAacac > 1:510584/1‑150 (MQ=255) cTTGATCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGc > 2:510481/1‑150 (MQ=255) aTCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAgg < 2:567968/150‑1 (MQ=255) tCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAggg > 2:276445/1‑150 (MQ=255) gCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCAACGCCGATGGCGTCatcaacg < 1:145977/139‑7 (MQ=255) gCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCAACGCCGATGGCGTCatcaacg > 2:145977/1‑133 (MQ=255) cTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGaa < 1:510481/150‑1 (MQ=255) aaTGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGc < 2:97385/150‑1 (MQ=255) aaTGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGACATCTGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGc < 1:267406/150‑1 (MQ=255) cccTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGttt < 1:486491/150‑1 (MQ=255) cTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCa > 1:418530/1‑150 (MQ=255) aCCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCt > 1:278732/1‑150 (MQ=255) cccGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCActgcctgc > 2:350479/1‑150 (MQ=255) cgtcgtGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTgcctgcct > 1:325266/1‑150 (MQ=255) cgtcgtGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTgcctgcct < 1:276445/150‑1 (MQ=255) gtGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCAGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTgcc < 1:384123/150‑1 (MQ=255) gCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCgta < 2:253656/150‑1 (MQ=255) aTCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCgtagta > 1:248653/1‑150 (MQ=255) aCCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGTGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCg < 1:150367/150‑1 (MQ=255) cacCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGaa < 2:418530/150‑1 (MQ=255) ccAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGga < 1:55549/132‑1 (MQ=255) ccAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGga > 2:55549/1‑132 (MQ=255) aGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGc > 1:94896/1‑80 (MQ=255) aGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGc < 2:94896/80‑1 (MQ=255) tgTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCATGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAGCTGGCTGACGTTGCTCACCt < 1:204141/149‑1 (MQ=255) aaCGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAGCTGGCTGACGTTGCTCACCTGGCCGGt > 1:317358/1‑150 (MQ=255) tgaCGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAGCTGGCTGACGTTGCTCACCTGGCCGGTACCGTTGt < 2:278732/150‑1 (MQ=255) tgaCGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAGCTGGCTGACGTTGCTCACCTGGCCGGTACCGTTGt < 2:510584/150‑1 (MQ=255) gaCGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAGCTGGCTGACGTTGCTCACCTGGCCGGTACCGTTGTc < 1:236231/150‑1 (MQ=255) aCGCCAACGCCGATGGCGTCATCACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAgc < 1:279727/114‑1 (MQ=255) aCGCCAACGCCGATGGCGTCATCACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAgc > 2:279727/1‑114 (MQ=255) | CTACCGGCTCGACCTGGCCGAGCTTGATCTGGCTAATGCCATGCTCCTGCAATGCCCTTAGCTCACCGGCATCGGCACGCCCGTCGTGGTTGCCATCGACCCACACCTTCAATTGCGCCCAGACGGCGTCTTCAACGTTGATGACGCCATCGGCGTTGGCGTCAACACTTGCCAGGGCGGCGAAGCCATCCTTGAACGGCGTTTCACCAGAGCTGCCATCACTGCCTGCCTTGCCGCCGTAGTATTCGGAGAACAGCTGGCTGACGTTGCTCACCTGGCCGGTACCGTTGTC > NC_002947/5602083‑5602374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |