| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,968,245 | C→T | 11.8% | intergenic (‑112/+23) | glnK ← / ← PP_5723 | NRII(GlnL/NtrB) phosphatase activator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,968,245 | 0 | C | T | 11.8% | 83.1 / 3.6 | 34 | intergenic (‑112/+23) | glnK/PP_5723 | NRII(GlnL/NtrB) phosphatase activator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (18/12); new base T (2/2); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
AGCTTGAACGGCTTGATGATGGCTGTGACTAGCTTCATGAAACTCTCTCCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTC > NC_002947/5968096‑5968393 | aGCTTGAACGGCTTGATGATGGCTGTGACTAGCTTCATGAAACTCTCTCCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCAc > 2:191323/1‑150 (MQ=255) gAACGGCTTGATGATGGCTGTGACTAGCTTCATGAAACTCTCTCCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCt > 1:309805/1‑150 (MQ=255) aCGGCTTGATGATGGCTGTGACTAGCTTCATGAAACTCTCTCCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTgg < 2:613295/150‑1 (MQ=255) tgtgACTAGCTTCATGAAACTCTCTCCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCtt < 1:359480/150‑1 (MQ=255) tGAAACTCTCTCCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCg > 2:462433/1‑150 (MQ=255) ctcCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCt < 2:309805/150‑1 (MQ=255) tcCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCtt > 2:145645/1‑150 (MQ=255) ccGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTg > 1:153628/1‑150 (MQ=255) gATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCa < 1:191323/150‑1 (MQ=255) gtggACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCAt < 1:330483/124‑1 (MQ=255) gtggACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCAt > 2:330483/1‑124 (MQ=255) ccccAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGtt > 1:127444/1‑150 (MQ=255) ccAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTcc > 1:433245/1‑150 (MQ=255) aGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCATTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGa > 2:590033/1‑150 (MQ=255) aGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCATTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGa > 2:589715/1‑150 (MQ=255) aaacCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAgcgc > 1:566856/1‑150 (MQ=255) aaacCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAgcgc < 2:566856/150‑1 (MQ=255) acCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCAt > 2:396135/1‑101 (MQ=255) acCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCAt < 1:396135/101‑1 (MQ=255) ccGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAg > 2:154152/1‑82 (MQ=255) ccGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAg < 1:154152/82‑1 (MQ=255) gTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAgcgc < 1:145645/150‑1 (MQ=255) tCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGcgcg > 1:259623/1‑150 (MQ=255) gcTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGcgcg > 2:155307/2‑150 (MQ=255) gTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGcc < 1:589934/124‑1 (MQ=255) gTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGcc > 2:589934/1‑124 (MQ=255) ggCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCg < 2:259623/150‑1 (MQ=255) gCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCATTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCgg < 1:590033/150‑1 (MQ=255) gCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCATTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCgg < 1:589715/150‑1 (MQ=255) tAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCGGGATTgg > 2:277708/1‑150 (MQ=255) aCGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACCGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGcc > 2:405582/1‑150 (MQ=255) gcgTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACCGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAg < 1:405582/150‑1 (MQ=255) gCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCa > 2:565020/1‑150 (MQ=255) gcgTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGc > 2:254531/1‑150 (MQ=255) aCTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTc > 2:27671/1‑150 (MQ=255) | AGCTTGAACGGCTTGATGATGGCTGTGACTAGCTTCATGAAACTCTCTCCCGATTTGGTGGACTTGCCCCAGGAAAACAAACCCGTCTCAAGTCTAAGCGCAGCGGTTGGCTTTGTAACGCGTCGTCGGCATCCGGCTCCGCGTGCGCACTGCCTGGTCACAAGGAACTGCTTCAGTGCATGGCTCGTACAGGTCTTTGCAGAAACCTTGCCAGTTCCGACAAAGCGCCGAAAAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTC > NC_002947/5968096‑5968393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |