| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,988,875 | T→C | 24.3% | intergenic (+113/‑433) | kefB‑III → / → PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,875 | 0 | T | C | 24.3% | 80.7 / 16.1 | 37 | intergenic (+113/‑433) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base T (11/17); new base C (6/3); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGC > NC_002947/5988728‑5989015 | agctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTgg < 1:356221/150‑1 (MQ=255) tggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GAc < 1:276171/150‑1 (MQ=255) gACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGc < 2:31159/150‑1 (MQ=255) ttttGGCGCGTGCCCTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGCTATcg > 1:205435/1‑150 (MQ=255) gcgcGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcg > 2:291262/1‑150 (MQ=255) ttGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATcgcg > 2:40863/1‑138 (MQ=255) ttGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATcgcg < 1:40863/138‑1 (MQ=255) tGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAg < 2:205435/150‑1 (MQ=255) gAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGTGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAgg < 1:580814/150‑1 (MQ=255) aTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTc > 2:473279/1‑134 (MQ=255) aTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTc < 1:473279/134‑1 (MQ=255) gcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggagg > 1:408450/1‑150 (MQ=255) gcgcgGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGG‑GATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGc < 2:358943/116‑1 (MQ=14) gcgcgGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGG‑GATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGc > 1:358943/1‑116 (MQ=17) ggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaa < 2:408450/150‑1 (MQ=255) ggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCCGGCACAGGAGGGATTGGCCAGaaa > 1:490680/1‑150 (MQ=255) gcgcTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGG‑GATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑Gaaaca > 2:539019/1‑150 (MQ=9) gcTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGG‑GATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑Gaaacaaa < 2:284445/150‑1 (MQ=9) gcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTa < 1:106963/150‑1 (MQ=255) gcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGG‑GATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAg < 2:88875/150‑1 (MQ=2) gcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGG‑GATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAg > 1:453488/1‑150 (MQ=2) ggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGa > 1:410273/1‑150 (MQ=255) cccGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGG‑GATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGtt > 2:336591/1‑150 (MQ=2) cccGCTCCTACGTTTGTTTCGGGCCAATCATTTCGCCGGTGAC‑CTGGCTGGGGTGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGTCTGGCC‑GAAACAAACGTAGGAGCGGGtt > 1:78205/1‑150 (MQ=9) gtttgtttCGGGCCAATTATTCCTGCGG‑GATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAACCGTAGAAGCGGGTTTACCCGCGCAg > 2:160213/1‑150 (MQ=2) tttgtttCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAg < 2:488234/81‑1 (MQ=255) tttgtttCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAg > 1:488234/1‑81 (MQ=255) ttgtttCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagc < 2:437236‑M2/150‑11 (MQ=255) tttCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcgt > 2:113153/1‑68 (MQ=255) tttCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcgt < 1:113153/68‑1 (MQ=255) tttCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgcc < 1:86568‑M2/150‑14 (MQ=255) gggCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgc < 1:534368‑M2/150‑18 (MQ=255) ggCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcg < 2:319394‑M2/150‑19 (MQ=255) cAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggc < 1:398383‑M2/150‑22 (MQ=255) ttATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggc > 2:398383‑M2/1‑126 (MQ=255) tATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaa < 2:111867/109‑1 (MQ=255) tATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaa > 1:111867/1‑109 (MQ=255) | AGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGG‑GACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGC > NC_002947/5988728‑5989015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |