Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	439,419	A→T	15.4%	N185K (AAT→AAA)	PP_0361 ←	competence protein ComF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	439,419	0	A	T	15.4%	97.4 / 11.3	39	N185K (AAT→AAA)	PP_0361	competence protein ComF
Reads supporting (aligned to +/- strand):  ref base A (15/18);  new base T (2/4);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

ATACACATCCACCCGCCGCGCACCTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAGGTTACCCCGACGTGCGACGACAACCACCGCCCAAGCATCCCCGCCTGGTT  >  NC_002947/439278‑439547                                                                                                                                               |                                                                                                                                 aTACACATCCACCCGCCGCGCACCTGCCTTGCGCAGTGTCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGcgccg                                                                                                                          <  1:31666/150‑1 (MQ=255)  tACACATCCACCCGCGGGCCACCTGGCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGG‑ACCAGAGTTCAGACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAATTTGcgccag                                                                                                                         <  1:346272/150‑3 (MQ=255)  tACACATCCACCCGCCGCGCACCTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTNCNTNCCAGTTCGCCCTGTNNCGCGAACGCCTGGCGCAATTTGcgccag                                                                                                                         <  1:416170/150‑3 (MQ=255)           aCCCGCCGCGCACCTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTg                                                                                                                >  1:26811/1‑150 (MQ=255)             ccgccgCGCACCTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGgc                                                                                                              >  2:537631/1‑150 (MQ=255)                      aCCTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTg                                                                                                                               <  2:297484/124‑1 (MQ=255)                      aCCTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTg                                                                                                                               >  1:297484/1‑124 (MQ=255)                      aCCTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATg                                                                                                     <  1:23935/150‑1 (MQ=255)                       ccTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAATTTGcgccag                                                                                                                         <  2:331084/129‑3 (MQ=255)                       ccTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAATTTGcgccag                                                                                                                         >  1:331084/1‑127 (MQ=255)                        cTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAATTTGcgccag                                                                                                                         >  1:189589/1‑126 (MQ=255)                        cTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAATTTGcgccag                                                                                                                         <  2:189589/128‑3 (MQ=255)                        cTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGtt                                                                                                   >  2:540407/1‑150 (MQ=255)                                     tGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGgtgt                                                                                      >  1:159094/1‑150 (MQ=255)                                       ccTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGcc                                                                                                                   >  1:237484/1‑119 (MQ=255)                                       ccTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGcc                                                                                                                   <  2:237484/119‑1 (MQ=255)                                         tGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGtc                                                                                     <  1:326257/147‑1 (MQ=255)                                         tGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGtc                                                                                     >  2:326257/1‑147 (MQ=255)                                         tGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAAGTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTcg                                                                                  >  1:194442/1‑150 (MQ=255)                                                  gCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCgg                                                                         <  1:36645/150‑1 (MQ=255)                                                           gTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCaaa                                                                >  2:118676/1‑150 (MQ=255)                                                               cACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCt                                                            >  1:433241/1‑150 (MQ=255)                                                                aCCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTc                                                           >  2:542002/1‑150 (MQ=255)                                                                  cGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCggg                                                                        >  1:323544/1‑135 (MQ=255)                                                                  cGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCggg                                                                        <  2:323544/135‑1 (MQ=255)                                                                   ggtggtCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGtt                                                        >  2:489306/1‑150 (MQ=255)                                                                   ggtggtCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGtt                                                        <  1:118676/150‑1 (MQ=255)                                                                      ggtCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCa                                                     <  2:7955/150‑1 (MQ=255)                                                                       gtCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGGCCAGATGTTGCTGTGCCTGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAg                                                    <  1:537631/150‑1 (MQ=255)                                                                       gtCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAg                                                    <  2:26811/150‑1 (MQ=255)                                                                       gtCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAg                                                    <  1:540407/150‑1 (MQ=255)                                                                       gtCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCAGTCCCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAg                                                    <  1:195006/150‑1 (MQ=255)                                                                                          aTGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAGGTTACCCCGACGTGcgac                                  <  1:489306/150‑1 (MQ=255)                                                                                                aCATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTg                                                                                                                >  2:343446/1‑66 (MQ=255)                                                                                                aCATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTg                                                                                                                <  1:343446/66‑1 (MQ=255)                                                                                                            tCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAGGTTACCCCGACGTGcgac                                  >  2:257472/1‑132 (MQ=255)                                                                                                            tCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAGGTTACCCCGACGTGcgac                                  <  1:257472/132‑1 (MQ=255)                                                                                                                      ccTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAGGTTACCCCGACGTGCGACGACAACCACCGCCCAAGCATCCCCGCCt      <  1:454598/150‑1 (MQ=255)                                                                                                                          tACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAGGTTACCCCGACGTGCGACGACAACCACCGCCCAAGCATCCCCGCCTGGtt  <  2:218202/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                 ATACACATCCACCCGCCGCGCACCTGCCTTGCGCAGTGCCTGGGCGATAGCCTGCGCAGTGGCACCGGTGGTCA‑ACACATCATCGACGATGGCGACATGCTTGCCTTCCAGTTCGCCCTGTACCGCGAACGCCTGGCGCAAATTGCGCCGCCGTGCCTTGGCGCCCAGATGTTGCTGTGCCGGTGTCTCGCGGGTGCGGGCCAGCAAACGCTCGTTGCAGGTTACCCCGACGTGCGACGACAACCACCGCCCAAGCATCCCCGCCTGGTT  >  NC_002947/439278‑439547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑