Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,027,279 A→T 12.9% F212I (TTC→ATC) ‡ PP_5277 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,027,2790AT12.9% 80.8 / 5.5 31F212I (TTC→ATC) ‡PP_5277MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (10/17);  new base T (2/2);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

CATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCG  >  NC_002947/6027136‑6027424
                                                                                                                                               |                                                                                                                                                  
cATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGGCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGc                                                                                                                                              <  2:239695/150‑1 (MQ=255)
    tGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTAAATTCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAAc                                                                                                                                          >  1:500566/1‑150 (MQ=255)
      gTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGTTCGAGGCGatgat                                                                                                                                        <  2:462248/150‑5 (MQ=255)
       tCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAgg                                                                                                                                       <  1:388388/150‑1 (MQ=255)
            cAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAAt                                                                                                                                  >  1:252598/1‑150 (MQ=255)
                       tGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGAGCAATGGCCCAGGCaa                                                                                                                       <  2:209825/150‑1 (MQ=255)
                             aTTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGAACATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCg                                                                                                                 <  1:418360/150‑1 (MQ=255)
                              ttGCAGGACCGCATCAGCCACCACCACGGTACTCACCATGAACCAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCgg                                                                                                                <  2:224985/150‑1 (MQ=255)
                              ttGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCgg                                                                                                                <  1:393771/150‑1 (MQ=255)
                                 cagcaCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCaa                                                                                                             <  2:112789/150‑1 (MQ=255)
                                     aCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCa                                                                                                                            <  2:395223/131‑1 (MQ=255)
                                     aCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCa                                                                                                                            >  1:395223/1‑131 (MQ=255)
                                                caccaccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGc                                                                                             >  1:249791/1‑150 (MQ=255)
                                                         ggTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTc                                                                                    <  1:512046/150‑1 (MQ=255)
                                                              tCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCaa                                                                               >  2:337765/1‑150 (MQ=255)
                                                                aCCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAAc                                                                                                                                          >  2:211838/1‑90 (MQ=255)
                                                                aCCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAAc                                                                                                                                          <  1:211838/90‑1 (MQ=255)
                                                                  cATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGt                                                                           >  1:503567/1‑150 (MQ=255)
                                                                        cAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACt                                                                     <  1:10205/150‑1 (MQ=255)
                                                                                                 tgctgcAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCCGGCGATTACCGGCAAGCGTCGGCAGA‑AGGCGAACATGGCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTcca                                            >  1:599466/1‑150 (MQ=255)
                                                                                                  gctgcAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTccac                                           <  1:337765/150‑1 (MQ=255)
                                                                                                                  cGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAAc                           <  2:252598/150‑1 (MQ=255)
                                                                                                                   ggCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAAcc                          <  2:500566/150‑1 (MQ=255)
                                                                                                                           ggTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAg                        <  2:165555/144‑1 (MQ=255)
                                                                                                                           ggTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAg                        >  1:165555/1‑144 (MQ=255)
                                                                                                                              gAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggcgcggc               <  2:249791/150‑1 (MQ=255)
                                                                                                                                 aatcatcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTGGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcag            >  2:421876/2‑150 (MQ=255)
                                                                                                                                   tgttcGCCTCGTTCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGgcgc                                                <  2:219196/109‑1 (MQ=255)
                                                                                                                                   tgttcGCCTCGTTCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGgcgc                                                >  1:219196/4‑112 (MQ=255)
                                                                                                                                     ttcGCCTCGTTCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcagc        >  2:440209/2‑150 (MQ=255)
                                                                                                                                           tCGAACGATGTGAACACGGCAATGGCCCAGGCAATTCCCGGCAAGCGTCCGAAGATCTGTAGGCCGCTC‑CTAAAGGTCACATTGCAGTCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCg  <  1:43961/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGA‑AGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCG  >  NC_002947/6027136‑6027424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑