| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 794,611 | G→A | 11.4% | L250L (CTG→CTA) | PP_0680 → | ATP‑dependent protease‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 794,611 | 0 | G | A | 11.4% | 85.7 / 3.1 | 35 | L250L (CTG→CTA) | PP_0680 | ATP‑dependent protease‑like protein |
| Reads supporting (aligned to +/- strand): ref base G (15/16); new base A (2/2); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01 | |||||||||||
GCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGATGACAGCAAGTCCGACGCCGTG > NC_002947/794464‑794746 | gCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCa < 2:131185/150‑1 (MQ=255) ggTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCt < 2:235155/150‑1 (MQ=255) aTTCCAAGAGGACACTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCCGGCTGTACAAGTGTAGTCGTGAAGCGAACAACCAGCTTCGCCCGCTCAACGACGCCCACTTCCTCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTc < 1:141652/150‑1 (MQ=255) aCGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTACAGCCGTTGCTGGCGCCGCTGTCGGaaa > 2:52693/1‑150 (MQ=255) gACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTAc < 2:388954/150‑1 (MQ=255) cccTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTa < 2:546214/142‑1 (MQ=255) cccTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTa > 1:546214/1‑142 (MQ=255) gaggagCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACgcggcg < 1:551768/150‑1 (MQ=255) aaCGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTAc > 1:144969/1‑150 (MQ=255) agagCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCa > 1:514509/1‑150 (MQ=255) cAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCa > 1:7574/1‑150 (MQ=255) cAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCa > 1:227820/1‑150 (MQ=255) cAGCCTGCCGCAACGGAAGCGCGAATCGAACAACCAGCTGCCCCAGCTCAACGGAGCAAACCTCCCACTGCCACTGCAGCCGTTGCCGGCGCCGCTGTCGGAAAAGTAAGCAGAAAACGCGACCGTCTTCGCCTACCTGCAATCGGTGcc > 2:43543/1‑149 (MQ=255) cTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTg > 1:203519/1‑150 (MQ=255) ccGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAAc < 1:545330/150‑1 (MQ=255) cAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTg > 2:43470/1‑150 (MQ=255) tGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACAATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTg > 1:383242/1‑147 (MQ=255) tGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACAATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTg < 2:383242/147‑1 (MQ=255) aTCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTACAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGt < 1:52693/150‑1 (MQ=255) cGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGACACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCg < 1:425654/150‑1 (MQ=255) aacaacCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAg > 2:349315/1‑150 (MQ=255) gcgcCAGATCAACGAAGAAGCCATCACCCTGGCACTGCAGCCGTGGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAAGCGGGGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGa < 2:7574/150‑1 (MQ=255) ccAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGATGa < 2:203519/150‑1 (MQ=255) cAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGATGAc > 2:465261/1‑150 (MQ=255) aGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGATGACa < 1:43470/150‑1 (MQ=255) gCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGATGACAg < 2:550014/150‑1 (MQ=255) aCGAAGAAACCATCACCCTGGCACTGCAGCCGTTCCGGGCGCCGCGGTCGGAAAAGTACGCCGAACACGCGGCGGTGTGCGCCTACCTGCAACCGGTGCAGTGGAACCTCTTGCGTACGCTGGTCGAGCAACTGGTCGATGACAGCAAGt > 2:310617/1‑150 (MQ=255) aaaCCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTg < 1:582154/128‑1 (MQ=255) aaaCCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTg > 2:582154/1‑128 (MQ=255) aCCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGATGACAGCAAGTCCGACGcc < 2:514509/150‑1 (MQ=255) aTCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGCAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGATGACCGCAAGTCCGACGCCGTg > 2:232334/1‑150 (MQ=255) cccTGGCACTACAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGa > 1:7387/1‑112 (MQ=255) cccTGGCACTACAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGa < 2:7387/112‑1 (MQ=255) cTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGc < 1:548037/38‑1 (MQ=255) cTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGc > 2:548037/1‑38 (MQ=255) | GCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGCGCCTACCTGCAATCGGTGCAGTTGAACCTGTTGCGTACGCTGGTCGAGCAACTGGTCGATGACAGCAAGTCCGACGCCGTG > NC_002947/794464‑794746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |