| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 878,918 | C→A | 15.8% | G148G (GGC→GGA) | PP_0763 → | medium‑chain‑fatty acid CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 878,918 | 0 | C | A | 15.8% | 60.0 / 3.0 | 25 | G148G (GGC→GGA) | PP_0763 | medium‑chain‑fatty acid CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (13/8); new base A (2/2); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GCCTGTCGCCCGAGCAGATCCTCTACACCATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCAGCCCGCGCTACGACTTCCCGGATTTCGACGAGAATTCGGTGGCCACTACCTTCTACACCACTGGCACCACCGGTAA > NC_002947/878776‑879058 | gcCTGTCGCCCGAGCAGATCCTCTACACCATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTAGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACa > 2:512089/1‑150 (MQ=255) tGTCGCCCGAGCAGATCCTCTACACCATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGa < 1:313861/150‑1 (MQ=255) cccGAGCAGATCCTCTACACCATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGcc < 1:613606/150‑1 (MQ=255) gAGCAGATCCTCTACACCATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGaa > 1:97661/1‑150 (MQ=255) ccATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACaa < 1:124821/124‑1 (MQ=255) ccATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACaa > 2:124821/1‑124 (MQ=255) cATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTAGCGGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGAACCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCgg > 1:419935/1‑150 (MQ=255) cGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGCCTGGTc < 1:466180/136‑1 (MQ=255) cGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGCCTGGTc > 2:466180/1‑136 (MQ=255) ccGCGTGGTGCTGGTCGTTAGTGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAgctgag < 2:419935/150‑3 (MQ=255) cgcgTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGa < 2:65306/134‑1 (MQ=255) cgcgTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGa > 1:65306/1‑134 (MQ=255) gTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGAACACTGTCGACAAGACCCTGCTACTGACCGGTGGCCCGTACAGGACTGCCTGACTGCCCGATCTGTGGTGGGTGTATGAGCAGCTGCTGGCTGCTGCCAGc > 2:130477/1‑150 (MQ=255) gtcgGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCAGCCCGCGCTACGACTTc > 2:481445/1‑150 (MQ=255) cTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCAGCCCGCGCTACGACTTCCCGGAt > 1:434206/1‑150 (MQ=255) tCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGATGTCTGCTGCCAGCCCGCGCTACGACTTCCCGGATTTCGACGAGAAtt > 1:67248/1‑150 (MQ=255) cACTGTCGACAAGACCCTGCTACTGACCGATGGACCGGACAAGACTGc > 2:12102/1‑48 (MQ=255) cACTGTCGACAAGACCCTGCTACTGACCGATGGACCGGACAAGACTGc > 2:321153/1‑48 (MQ=255) cACTGTCGACAAGACCCTGCTACTGACCGATGGACCGGACAAGACTGc < 1:321153/48‑1 (MQ=255) cACTGTCGACAAGACCCTGCTACTGACCGATGGACCGGACAAGACTGc < 1:12102/48‑1 (MQ=255) tGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCAGCCCGCGCTACGACTTCCCGGATTTCGACGAGAATTCGGTGGCCACTACCTTCTa < 1:512089/150‑1 (MQ=255) gACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCAGCCCGCGCTACGACTTCCCGGATTTCGACGAGAATTCGGTGGCCACTACCTTCTAcacc > 2:470132/1‑150 (MQ=255) gACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCa > 1:234884/1‑74 (MQ=255) gACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCa < 2:234884/74‑1 (MQ=255) gACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCAGCCCGCGCTACGACTTCCCGGATTTCGACGAGAATTCGGTGGCCACTACCTTCTACACCACTGGCACCACCGGTaa > 2:66217/1‑150 (MQ=255) | GCCTGTCGCCCGAGCAGATCCTCTACACCATGAACCATGCCGAAGACCGCGTGGTGCTGGTCAACAGCGACTTCGTCGGCCTGTACCAGGCCATCGCCGGGCAGCTGACCACTGTCGACAAGACCCTGCTACTGACCGATGGCCCGGACAAGACTGCCGAACTGCCCGGTCTGGTCGGCGAGTATGAGCAGCTGCTGGCTGCTGCCAGCCCGCGCTACGACTTCCCGGATTTCGACGAGAATTCGGTGGCCACTACCTTCTACACCACTGGCACCACCGGTAA > NC_002947/878776‑879058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |