Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,033,816	T→A	12.7%	F145I (TTC→ATC)	PP_0891 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,033,816	0	T	A	12.7%	79.7 / 5.1	32	F145I (TTC→ATC)	PP_0891	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/12);  new base A (2/2);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATC  >  NC_002947/1033675‑1033950                                                                                                                                              |                                                                                                                                       gCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtg                                                                                                                                >  2:99566/1‑150 (MQ=255) gCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtg                                                                                                                                <  2:207899/150‑1 (MQ=255)   acaGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtggt                                                                                                                              >  1:360444/1‑150 (MQ=255)        tCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGAccc                                                                                                                         <  2:360444/150‑1 (MQ=255)           aCCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTg                                                                                                                      >  1:482714/1‑150 (MQ=255)            ccTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGa                                                                                                                     <  1:377693/150‑1 (MQ=255)                    cggtCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTgg                                                                                                             >  1:384730/4‑150 (MQ=255)                                  gCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGaa                                                                                               <  1:77344/150‑1 (MQ=255)                                    cGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGa                                                                                             <  2:265349/150‑1 (MQ=255)                                    cGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGa                                                                                             >  2:18259/1‑150 (MQ=255)                                             acaGATCGAATTTCGCCTGCCGCTGACCGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTTGCCACCCGCCTGGATGTGCGACGCTTCCAGGTGGTGACCCTTGATCCTCTGGTGCTGATCGCCGAAGATTATGGCtt                                                                                    >  2:281397/1‑150 (MQ=255)                                             acaGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCtt                                                                                    <  1:99566/150‑1 (MQ=255)                                                                   cTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTg                                                              <  2:11498/150‑1 (MQ=255)                                                                           aaCCCGGCATGGGCAGTCGCACAGCTACAATGTCCTGTTGCTGGCAACCCGACTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGTGGCTGGAAAGCCTGCGCAAGtt                                                      >  2:277446/2‑150 (MQ=255)                                                                              ccTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGATCCtt                                                                                                                       >  1:292130/1‑82 (MQ=255)                                                                              ccTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCtt                                                                                                                       <  2:292130/82‑1 (MQ=255)                                                                                 gCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAg                                                >  1:61839/1‑150 (MQ=255)                                                                                        cAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCGGGATGCGCGGCGCTCCGAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCCTGCTGCCGGGGCTGTGAAGCCTGCGCAAGTTCGCCAGGTTGAAg                                         >  2:245818/1‑150 (MQ=255)                                                                                            cGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCa                                     <  1:548456/150‑1 (MQ=255)                                                                                            cGCACAGCTACAATGTCCTGTTGCTGGAATTTCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCa                                     <  1:463402/150‑1 (MQ=255)                                                                                             gCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCAt                                    <  2:433645/150‑1 (MQ=255)                                                                                                              tGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                         <  1:464966/112‑1 (MQ=255)                                                                                                              tGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                         >  2:464966/1‑112 (MQ=255)                                                                                                               gTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGt                  >  1:597844/1‑150 (MQ=255)                                                                                                                   cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAgc              >  1:390445/1‑150 (MQ=255)                                                                                                                          cccGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGt                            >  2:162650/1‑129 (MQ=255)                                                                                                                          cccGCCTGGATGCGCGGCGCGTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGt                            <  1:162650/129‑1 (MQ=255)                                                                                                                            caccTGGAAGCGCCGCGCATCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGt                                        <  2:403383/113‑1 (MQ=255)                                                                                                                            caccTGGAAGCGCCGCGCATCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGt                                        >  1:403383/3‑115 (MQ=255)                                                                                                                              ccTGGAAGCGCCGCGCATCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGa    >  2:466488/1‑149 (MQ=255)                                                                                                                              ccTGGAAGCGCCGCGCATCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGa    <  1:466488/149‑1 (MQ=255)                                                                                                                               cTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATc  >  2:387452/1‑150 (MQ=255)                                                                                                                                              |                                                                                                                                       GCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATC  >  NC_002947/1033675‑1033950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑