| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,948,680 | Δ1 bp | 27.3% | coding (213/435 nt) | fliO ← | flagellar protein FliO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,948,680 | 0 | C | . | 27.3% | 77.7 / 30.1 | 33 | coding (213/435 nt) | fliO | flagellar protein FliO |
| Reads supporting (aligned to +/- strand): ref base C (11/13); new base . (5/4); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
GGCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCC > NC_002947/4948540‑4948815 | ggCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCa < 2:198915/150‑1 (MQ=255) cACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCAcc < 2:261994/150‑1 (MQ=255) ttCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGt < 1:384689/150‑1 (MQ=255) gCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGctct > 2:19880/1‑150 (MQ=255) gTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCa > 1:514856/1‑150 (MQ=255) tACGTGGACGGCCTCGCTGGTGCCGAGCCTATGGGCGATCAGGATCTCCCCCTTGCCAACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAg < 2:113386/150‑1 (MQ=255) gggCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCgca < 2:514856/150‑1 (MQ=255) tCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACGATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAgcc > 2:4707/1‑150 (MQ=255) gATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGcc > 2:601236/1‑150 (MQ=255) ccTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagca > 2:542110/1‑150 (MQ=255) cTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagcag > 1:120846/1‑150 (MQ=255) ccACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAgg > 2:283024/1‑128 (MQ=255) ccACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAgg < 1:283024/128‑1 (MQ=255) ccACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagcag < 2:5095/144‑1 (MQ=255) ccACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagcag > 1:5095/1‑144 (MQ=255) agcagcagGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTgc > 1:17294/1‑150 (MQ=255) cGGTCACGCGGGCTGATGGCACGGCGGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGcc < 1:80811/150‑1 (MQ=255) gTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGccc < 1:542110/150‑1 (MQ=255) gTCACGCGGGCCGATGGCACGGCTCGGTGCAAGCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCg > 1:323531/1‑150 (MQ=255) gggCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCtt < 2:72168/47‑1 (MQ=255) gggCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCtt > 1:72168/1‑47 (MQ=255) gggCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGa < 2:120846/150‑1 (MQ=255) cACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGtt < 2:17294/150‑1 (MQ=255) aCGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTg > 1:73278/1‑150 (MQ=255) aCGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGa < 1:19880/150‑1 (MQ=255) cTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCCGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAACATCAGGCCCAGCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAgcc < 2:183068/150‑1 (MQ=255) ccGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAgccgcc > 1:516863/1‑150 (MQ=255) ccGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGtt < 2:596100/141‑1 (MQ=255) ccGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGtt > 1:596100/1‑141 (MQ=255) cGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGtt > 2:241745/1‑141 (MQ=255) cGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGtt < 1:241745/141‑1 (MQ=255) aTCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCaa > 2:113813/1‑98 (MQ=255) aTCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCaa < 1:113813/98‑1 (MQ=255) | GGCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAGGCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCC > NC_002947/4948540‑4948815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |