| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 634,115 | Δ1 bp | 21.1% | coding (418/2016 nt) | PP_0546 → | sigma‑54 dependent transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 634,115 | 0 | G | . | 21.1% | 43.7 / 9.6 | 19 | coding (418/2016 nt) | PP_0546 | sigma‑54 dependent transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (5/10); new base . (2/2); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
GGTGCTGCTGACCGACGCCCGCGGGGTGATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGC > NC_002947/633967‑634262 | ggTGCTGCTGACCGACGCCCGCGGGGTGATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGa < 2:26765/150‑1 (MQ=255) tgctgcTGACCGACGCCCGCGGGGTGATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAgc < 2:516343/150‑1 (MQ=255) ggggTGATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCATCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GAccatccat < 2:368634/150‑1 (MQ=255) gATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGaa < 1:31316/150‑1 (MQ=255) tCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGACTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTg > 2:167101/1‑150 (MQ=255) gTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGcc > 2:546085/1‑150 (MQ=255) gTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGcc < 2:199026/150‑1 (MQ=255) gAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCggg < 2:487382/150‑1 (MQ=255) gcgcAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTC‑AGCGCCTGGCGCTCGAcca > 1:404616/1‑107 (MQ=255) gcgcAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTC‑AGCGCCTGGCGCTCGAcca < 2:404616/107‑1 (MQ=255) cTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTc > 2:264471/1‑150 (MQ=255) aGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTC‑AGCGCCTGGCGCTCGAccagacat < 1:15075/101‑6 (MQ=38) aGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTC‑AGCGCCTGGCGCTCGAccagacat > 2:15075/1‑96 (MQ=38) aGGGCACCAATGGCATCGGCAAATGTGCGTTCGAGCGCCAGGCGCT‑GAACATACATCAGAACTAGCATTTTCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACg < 1:167101/150‑1 (MQ=255) gggCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACgt < 1:436867/150‑1 (MQ=255) gCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGACAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACgtgt > 2:323928/1‑150 (MQ=255) cACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTc > 2:217383/1‑150 (MQ=255) cACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTACTCGACGTGTc < 1:523748/150‑1 (MQ=255) tCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGc < 2:401789/150‑1 (MQ=255) | GGTGCTGCTGACCGACGCCCGCGGGGTGATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGC > NC_002947/633967‑634262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |