| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,088,283:1 | +A | 26.7% | coding (1212/1347 nt) | aroH ← | 3‑deoxy‑7‑phosphoheptulonate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,088,283 | 1 | . | A | 26.7% | 20.3 / 12.9 | 15 | coding (1212/1347 nt) | aroH | 3‑deoxy‑7‑phosphoheptulonate synthase |
| Reads supporting (aligned to +/- strand): ref base . (6/5); new base A (2/2); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01 | |||||||||||
AGCGAAAAGCGCTTAGCGCCGTACCTGCTTGAGGGTTTCGGCAATCAGGAACGCCAACTCCAGCGACTGATCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACGCATCCTCGGTGATCGGCCGGGCACCACCG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCAAAATCGCGGGTCTTGTAACCGCTGCTGGCCTTGAT > NC_002947/2088136‑2088429 | agcgaaaagcgCTTAGCGCCGTACCTGCTTGAGGGTTTCGGCAATCAGGAACGCCAACTCCAGCGACTGATCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACACATCCTCGGTGATCGGCCGGGCACCACCG‑At < 1:16886/150‑1 (MQ=255) ccTGCTTGAGGGTTTCGGCAATCAGGAACGCCAACTCCAGCGACTGATCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACGCATCCTCGGTGATCGGCCGGGCACCACCG‑ATGCATTCGGTGACGTTCTGCCCAg > 2:431852/1‑150 (MQ=255) gTTTCGGCAATCAGGAACGCCAACTCCAGCGACTGATCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACGCATCCTCGGTGATCGGACGGGCACCACCGAATGCA‑TCGGTggt > 1:538088/1‑126 (MQ=255) gTTTCGGCAATCAGGAACGCCAACTCCAGCGACTGATCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACGCATCCTCGGTGATCGGACGGGCACCACCGAATGCA‑TCGGTggt < 2:538088/128‑3 (MQ=255) tGATCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACGCATCCTCGGTGATCGGCCGGGCACCACCG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGGCCTCGGCCggg > 2:417578/1‑150 (MQ=255) aTCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACGCATCCTCGGTGATCGGCCGGGCACCACCG‑ATGCATTCGGt > 1:379818/1‑90 (MQ=255) aTCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACGCATCCTCGGTGATCGGCCGGGCACCACCG‑ATGCATTCGGt < 2:379818/90‑1 (MQ=255) gACAACGCATCCTCGGTGATCGGCCGGGCACCACCG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCaaaa > 2:364906/1‑150 (MQ=255) aCGCATCCTCGGTGATCGGCCGGGCACCACCG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCAAAATcgc < 1:431852/150‑1 (MQ=255) tCGGCCGGGCACCACCG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCAAAATCGCGGGTCTTGTAACCgc > 1:337230/1‑150 (MQ=255) tcaccGAATGCA‑TCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCAAAATCGCGGGTCtt > 1:129509/2‑131 (MQ=255) tcaccGAATGCA‑TCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCAAAATCGCGGGTCtt < 2:129509/130‑1 (MQ=255) caccG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCAAAATCGCGGGTCTTGTAACCGCTGCTGGCCTTGa > 2:269781/1‑150 (MQ=255) accG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCAAAATCGCGGGTCTTGTAACCGCTGCTGGCCTTGAt < 1:364906/150‑1 (MQ=255) accG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGCCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTTACGTAGAATCTGCGCAAAATCGCGGGTCTTGTAACCGCTGCTGGCCTTGAt < 1:417578/150‑1 (MQ=37) | AGCGAAAAGCGCTTAGCGCCGTACCTGCTTGAGGGTTTCGGCAATCAGGAACGCCAACTCCAGCGACTGATCGGCATTCATCCGCGGATCACAATGGGTGTGATACCGGTCAGACAACGCATCCTCGGTGATCGGCCGGGCACCACCG‑ATGCATTCGGTGACGTTCTGCCCAGTCATCTCGATATGGATACCGCCGGCGTGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACTGCTTCACCTCATCCAGAATCTGCGCAAAATCGCGGGTCTTGTAACCGCTGCTGGCCTTGAT > NC_002947/2088136‑2088429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |