| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 634,128:1 | +C | 22.2% | coding (431/2016 nt) | PP_0546 → | sigma‑54 dependent transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 634,128 | 1 | . | C | 22.2% | 41.6 / 12.4 | 18 | coding (431/2016 nt) | PP_0546 | sigma‑54 dependent transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (5/9); new base C (2/2); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
GGGGTGATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGT > NC_002947/633989‑634271 | ggggTGATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCATCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GAccatccat < 2:368634/150‑1 (MQ=255) gATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGaa < 1:31316/150‑1 (MQ=255) tCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGACTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTg > 2:167101/1‑150 (MQ=255) gTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGcc > 2:546085/1‑150 (MQ=255) gTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGcc < 2:199026/150‑1 (MQ=255) gAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCggg < 2:487382/150‑1 (MQ=255) gcgcAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTC‑AGCGCCTGGCGCTCGAcca > 1:404616/1‑107 (MQ=255) gcgcAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTC‑AGCGCCTGGCGCTCGAcca < 2:404616/107‑1 (MQ=255) cTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTc > 2:264471/1‑150 (MQ=255) aGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTC‑AGCGCCTGGCGCTCGAccagacat < 1:15075/101‑6 (MQ=38) aGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTC‑AGCGCCTGGCGCTCGAccagacat > 2:15075/1‑96 (MQ=38) aGGGCACCAATGGCATCGGCAAATGTGCGTTCGAGCGCCAGGCGCT‑GAACATACATCAGAACTAGCATTTTCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACg < 1:167101/150‑1 (MQ=255) gggCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACgt < 1:436867/150‑1 (MQ=255) gCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGACAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACgtgt > 2:323928/1‑150 (MQ=255) cACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTc > 2:217383/1‑150 (MQ=255) cACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTACTCGACGTGTc < 1:523748/150‑1 (MQ=255) tCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGc < 2:401789/150‑1 (MQ=255) aGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGt < 2:53320/150‑1 (MQ=255) | GGGGTGATCCTCAATTGCGTCAGTGCCCCCGCCGAGCGGCGCAGCTTCGAGCGTGCCGGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCT‑GACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGT > NC_002947/633989‑634271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |