| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 70 | C→T | 21.6% | intergenic (–/+77) | – / ← parB | –/chromosome‑partitioning protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 70 | 0 | C | T | 21.6% | 38.6 / 6.5 | 23 | intergenic (–/+77) | –/parB | –/chromosome‑partitioning protein |
| Reads supporting (aligned to +/- strand): ref base C (9/9); new base T (2/3); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGCGGATGTGAGCGAGCACGCCTTGCAACTCGTCAAGCGAGTTGTAGCGAATAACCAACTGGCCTTTGCC > NC_002947/1‑218 | acaaattgagccagcagtaccggaaaaaccgcccagcgatggaaaggcaggcggtttggcgtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGGCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCaga < 2:441742‑M1/73‑1 (MQ=255) tgagccagcagtaccggaaaaaccgcccagcgatggaaaggcaggcggtttggcgtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGTAGAGTATAg > 2:549546‑M1/72‑150 (MQ=255) ccgcccagcgatggaaaggcaggcggtttggcgtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCaa > 1:403748‑M1/50‑150 (MQ=255) cagcgatggaaaggcaggcggtttggcgtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCACGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCtt > 1:424693‑M1/45‑150 (MQ=255) aggcggtttggcgtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGa < 1:334381‑M1/86‑1 (MQ=255) aggcggtttggcgtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGa > 2:334381‑M1/30‑115 (MQ=255) aggcggtttggcgtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGAACGCATGGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATttctg < 1:591683‑M1/121‑3 (MQ=255) gcgtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACACCATGCTCGTAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCt < 1:194434‑M1/131‑1 (MQ=255) gtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAggg > 2:466981‑M1/18‑98 (MQ=255) gtgcgtatttccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAggg < 1:466981‑M1/81‑1 (MQ=255) tccatcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAAt > 1:409307‑M1/9‑150 (MQ=255) tcacAACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGtt > 2:367069‑M1/5‑150 (MQ=255) aaCTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAg < 2:169118/150‑1 (MQ=255) aCTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGc < 2:574716/150‑1 (MQ=255) tcctcGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGTAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGCGGAtg > 1:165857/1‑150 (MQ=255) aaGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAAcc < 1:48520/67‑1 (MQ=255) aaGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAAcc > 2:48520/1‑67 (MQ=255) aGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGTAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGCGGATGTGAGCGAGCACGCCTTGCaa < 2:194434/150‑1 (MQ=255) gTCAGCTATGACTTGGCATAATTTGTGCCAACAAAATTCGCGCATATTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGCGGATGTGAGCGAGCACGCCTTGCAAc < 1:441742/150‑1 (MQ=255) cAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGTAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGCGGATGTGAGCGAGCACGCCTTGCAACTc < 2:165857/150‑1 (MQ=255) aGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTacaa < 1:564344/105‑1 (MQ=255) aGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTacaa > 2:564344/1‑105 (MQ=255) gACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGCGGATGTGAGCGAGCACGCCTTGCAACTCGTCAAGCGAGTTGTAGCGAATAACCaa > 2:292901/1‑150 (MQ=255) gcAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGCGGATGTGAGCGAGCACGCCTTGCAACTCGTCAAGCGAGTTGTAGCGAATAACCAACTGGCCTTTGcc > 1:192590/1‑150 (MQ=255) | ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AACTGCTCCTCGGAAGTCGACCAACAAGTCAGCTATGACTTGGCATAATTTGTGCCGACAAAATGCGCGCAGAGTATAGGGGTGGATTAACCCCTATTCAACTCTTCGGTAGTGATTTCCGACTTCACGCTACAACAGGAATTGTTTCAGCGGATGTGAGCGAGCACGCCTTGCAACTCGTCAAGCGAGTTGTAGCGAATAACCAACTGGCCTTTGCC > NC_002947/1‑218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |