Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 150,828 A→C 9.6% L41R (CTG→CGG)  PP_0142 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947150,8280AC 9.6% 92.7 / 2.7 38L41R (CTG→CGG) PP_0142ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (13/21);  new base C (2/2);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CAGCGCCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATT  >  NC_002947/150680‑150955
                                                                                                                                                    |                                                                                                                               
cagcGCCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGTGGGCCCAGCTGGCTGAGTTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAg                                                                                                                                <  1:382014/150‑1 (MQ=255)
      ctggctggCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGcgctc                                                                                                                          >  1:538460/1‑150 (MQ=255)
      ctggctggCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGcgctc                                                                                                                          <  1:445883/150‑1 (MQ=255)
           tggctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCCTGcgctcgcatt                                                                                                                     <  2:87427/150‑4 (MQ=255)
              ctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTcgc                                                                                                                  >  2:452540/1‑150 (MQ=255)
               ttCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCTGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTcgcg                                                                                                                 >  2:50169/1‑150 (MQ=255)
                   ggCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCCGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTg                                                                                                             >  2:299908/1‑150 (MQ=255)
                     cAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAg                                                                                                           <  2:220058/150‑1 (MQ=255)
                     cAGCGCGTGGGTGCAGTGCAACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAg                                                                                                           <  2:172575/150‑1 (MQ=255)
                         gcgTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTg                                                                                                       <  2:148270/150‑1 (MQ=255)
                                tGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAgt                                                                                                >  1:297329/1‑150 (MQ=255)
                                     tGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGCCAGGGCAATGCGAGCGCCGGCGCTCGCCCTCGCGCTTGAGGTTGGCGTCGTGTGcc                                                                                           >  2:163621/1‑150 (MQ=255)
                                             gcgcTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCa                                                                                   <  2:538460/150‑1 (MQ=255)
                                             gcgcTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCa                                                                                   <  1:60184/150‑1 (MQ=255)
                                                                    ccAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgca                                                                  <  1:4798/144‑1 (MQ=255)
                                                                    ccAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgca                                                                  >  2:4798/1‑144 (MQ=255)
                                                                         agcGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTa                                                                                                  <  2:21866/107‑1 (MQ=255)
                                                                         agcGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTa                                                                                                  >  1:21866/1‑107 (MQ=255)
                                                                            gAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAg                                                                                                                                <  2:102509/74‑1 (MQ=255)
                                                                            gAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAg                                                                                                                                >  1:102509/1‑74 (MQ=255)
                                                                             aaGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCTACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGgc                                                   >  2:398295/1‑150 (MQ=255)
                                                                             aaGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGgc                                                   <  1:79166/150‑1 (MQ=255)
                                                                                 gcCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGgctgct                                               <  1:289134/150‑1 (MQ=255)
                                                                                     gCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgc                                                                   <  2:4594/126‑1 (MQ=255)
                                                                                     gCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgc                                                                   >  1:4594/1‑126 (MQ=255)
                                                                                         tgtCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCa                                       <  2:585140/150‑1 (MQ=255)
                                                                                                      cAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTGGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGACGGTTGGCTGCTGGGGTCCAGGGTGGCGCTggg                          <  1:163621/150‑1 (MQ=255)
                                                                                                           ggctgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTgg                     <  2:225656/150‑1 (MQ=255)
                                                                                                            gctgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTg                            <  2:116394/142‑1 (MQ=255)
                                                                                                            gctgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTg                            >  1:116394/1‑142 (MQ=255)
                                                                                                             ctgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGGGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGTGGGCGCTGGGGTTGGTc                   <  1:50169/150‑1 (MQ=255)
                                                                                                              tgAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCa                  >  1:472742/1‑150 (MQ=255)
                                                                                                                    cGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCGCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCt            <  2:45324/150‑1 (MQ=255)
                                                                                                                      gCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTcc          <  1:452540/150‑1 (MQ=255)
                                                                                                                      gCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTcc          >  1:393618/1‑150 (MQ=255)
                                                                                                                              gtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAAtt  <  2:472742/150‑1 (MQ=255)
                                                                                                                                            gCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTggg                          >  1:552851/1‑112 (MQ=255)
                                                                                                                                            gCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTggg                          <  2:552851/112‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                               
CAGCGCCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATT  >  NC_002947/150680‑150955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑