| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,526,622 | T→A | 11.7% | I256N (ATT→AAT) | ddlB → | D‑alanine‑‑D‑alanine ligase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,526,622 | 0 | T | A | 11.7% | 94.4 / 4.5 | 34 | I256N (ATT→AAT) | ddlB | D‑alanine‑‑D‑alanine ligase B |
| Reads supporting (aligned to +/- strand): ref base T (16/14); new base A (2/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
CCGCCGATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAGCAACT > NC_002947/1526474‑1526769 | ccgccgATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCAtt > 1:365283/1‑150 (MQ=255) cccTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCAATGGCATCGCAg < 1:270084/150‑1 (MQ=255) gggTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTg > 2:597363/1‑150 (MQ=255) gTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTggg < 1:504134/150‑1 (MQ=255) cGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCa < 2:134865/150‑1 (MQ=255) gACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCGGCGATGCCATTGGAATCGAAGGCTGGGGGGGGTTGGACGAGAtcgga > 2:225242/1‑146 (MQ=255) gACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAg < 2:137962/150‑1 (MQ=255) gCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAg > 1:22818/1‑115 (MQ=255) gCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAg < 2:22818/115‑1 (MQ=255) gCCAAGTACAGCGTCAAAGACCCCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAg < 2:599179/150‑1 (MQ=255) aaGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGc > 2:605837/1‑150 (MQ=255) aCATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGt < 2:180489/150‑1 (MQ=255) gATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGc < 2:34097/130‑1 (MQ=255) gATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGc > 1:34097/1‑130 (MQ=255) gTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAgg > 2:472630/1‑150 (MQ=255) tCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGcc > 1:163169/1‑150 (MQ=255) cAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCt < 2:365283/150‑1 (MQ=255) aaGGAACAGGAACTGATCGACCTGACCGCGCGCGCGTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGa < 2:147045/136‑1 (MQ=255) aaGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGa > 1:147045/1‑136 (MQ=255) aggaacaggaacTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTgg > 2:393624/1‑150 (MQ=255) caggaacTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGccc < 1:605837/150‑1 (MQ=255) aggaacTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCa > 1:34873/1‑150 (MQ=255) ggaacTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGGATCGAAGGCTTGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCAt > 1:261541/1‑150 (MQ=255) gaacTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGCGCGGTTGGACGTGATGGTTTACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATg > 1:381306/1‑150 (MQ=255) aacTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATgg < 2:34873/150‑1 (MQ=255) cTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGa < 1:369576/124‑1 (MQ=255) cTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGa > 2:369576/1‑124 (MQ=255) gACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGaa < 2:327601/96‑1 (MQ=255) gACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGaa > 1:327601/1‑96 (MQ=255) aCCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTg > 2:326657/1‑150 (MQ=255) gACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGc < 1:472630/150‑1 (MQ=255) ccTTCGATGCCAATGGCATCGCAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACt > 2:504206/1‑150 (MQ=255) ttcGATGCCAATGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATgg > 2:328820/3‑124 (MQ=255) ttcGATGCCAATGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATgg < 1:328820/122‑1 (MQ=255) cATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAGCAACt > 1:82425/1‑150 (MQ=255) | CCGCCGATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGCGCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAGCAACT > NC_002947/1526474‑1526769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |