Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,534,940	A→G	9.1%	intergenic (+89/‑94)	secA → / → argJ	protein translocase subunit SecA/glutamate N‑acetyltransferase/amino acid acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,534,940	0	A	G	9.1%	120.0 / 3.2	44	intergenic (+89/‑94)	secA/argJ	protein translocase subunit SecA/glutamate N‑acetyltransferase/amino acid acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (24/16);  new base G (2/2);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

TTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGG  >  NC_002947/1534809‑1535079                                                                                                                                    |                                                                                                                                            ttCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTAAACACACGTCTGTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTtgtg                                                                                                                           >  1:564912/1‑150 (MQ=255)      gCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGggc                                                                                                                      >  2:395763/1‑150 (MQ=255)        aagaagTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCTTTGTGGGgtcgg                                                                                                                    >  2:307868/1‑150 (MQ=255)        aagaagTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGggcgg                                                                                                                    >  1:522279/1‑150 (MQ=255)              ttCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTTCATGGACGAGcgcgg                                                                                                                                   >  2:464030/1‑128 (MQ=39)              ttCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTTCATGGACGAGcgcgg                                                                                                                                   <  1:464030/129‑2 (MQ=39)                 aaGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTc                                                                                                           <  1:287560/150‑1 (MQ=255)                      ttGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAGCGCGGTACCTGTGGGAGCGGCCTTGTGTTGCGaa                                                                                                      <  2:368236/150‑2 (MQ=14)                                 aGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGgcgc                                                                                                                                     <  1:131934/108‑1 (MQ=255)                                 aGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGgcgc                                                                                                                                     >  2:131934/1‑108 (MQ=255)                                               gAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCg                                                                             >  2:487060/1‑150 (MQ=255)                                                aaCCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGa                                                                            >  2:396792/1‑150 (MQ=255)                                                    ttACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGAcatc                                                                        >  2:86029/1‑150 (MQ=255)                                                       cGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACatcatc                                                                     <  1:396792/150‑1 (MQ=255)                                                                 gCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTa                                                           >  2:133798/1‑150 (MQ=255)                                                                      gggTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGggcg                                                                                                                     <  2:425773/87‑1 (MQ=255)                                                                      gggTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGggcg                                                                                                                     >  1:425773/1‑87 (MQ=255)                                                                           gCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGctct                                                 >  1:548833/1‑150 (MQ=255)                                                                                  ggCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCt                                          <  1:378682/150‑1 (MQ=255)                                                                                   gcgcTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTg                                         >  1:609948/4‑150 (MQ=255)                                                                                     ccTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATACTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGtt                                       >  2:453228/1‑150 (MQ=255)                                                                                           gCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttggtctt                                 <  2:522279/150‑1 (MQ=255)                                                                                            cAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATAGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttggtcttg                                >  1:38989/1‑150 (MQ=255)                                                                                                acaAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGggc                                                                                                                      >  2:585307/1‑60 (MQ=255)                                                                                                acaAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGggc                                                                                                                      <  1:585307/60‑1 (MQ=255)                                                                                                    ggCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCt                                                            <  1:382932/114‑1 (MQ=255)                                                                                                    ggCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCt                                                            >  2:382932/1‑114 (MQ=255)                                                                                                    ggCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCTCTCTAATGGCTGTTGGTCTTGGTCCTTTg                        <  2:564912/150‑1 (MQ=255)                                                                                                     gCCGCTCCCACAGGTACCGCGCTCGTCCATGATCGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAg                                                          <  1:194381/115‑1 (MQ=255)                                                                                                     gCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAg                                                          >  2:194381/1‑115 (MQ=255)                                                                                                     gCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGc                       >  1:582737/1‑150 (MQ=255)                                                                                                       cGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGccc                     >  1:114659/1‑150 (MQ=255)                                                                                                            ccACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAAt                                              <  1:244903/120‑1 (MQ=255)                                                                                                            ccACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAAt                                              >  2:244903/1‑120 (MQ=255)                                                                                                                aaggatcgcgCCCGTTCATGGACGAGCGCGGTACCTGTGGGAGCGGCCTTGTGTTGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCAc            >  2:389622/7‑150 (MQ=21)                                                                                                                   tACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttggtcttgg                               >  2:502102/1‑128 (MQ=255)                                                                                                                   tACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttggtcttgg                               <  1:502102/128‑1 (MQ=255)                                                                                                                      cgcgCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCTACATCATCGTTTTTTCGATGAGCGCTCTAATGTCTTTTGGTCTGGGGCCTTTGCCCACCCTGCACCCGCtt      >  2:585928/1‑150 (MQ=255)                                                                                                                       gcgcTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATgg                                            >  2:491583/1‑111 (MQ=255)                                                                                                                       gcgcTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATgg                                            <  1:491583/111‑1 (MQ=255)                                                                                                                       gcgcTCGTCCATGAACGGGCGCGATCCTTGTGGGAACGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTc     >  1:424019/1‑150 (MQ=255)                                                                                                                        cgcTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTcc    <  2:548833/150‑1 (MQ=255)                                                                                                                         gcTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCg   <  1:133798/150‑1 (MQ=255)                                                                                                                          cTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCgg  <  2:582737/150‑1 (MQ=255)                                                                                                                                    |                                                                                                                                            TTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGG  >  NC_002947/1534809‑1535079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑