Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,550,520	C→A	24.1%	A293D (GCT→GAT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,550,520	0	C	A	24.1%	24.5 / 3.0	16	A293D (GCT→GAT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base C (5/7);  new base A (2/2);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

ACTGCTGATCGTTGCCGAAGACGTTGAAGGTGAAGCGCTGGCTACCCTGGTAGTCAACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCG  >  NC_002947/1550380‑1550663                                                                                                                                             |                                                                                                                                                aCTGCTGATCGTTGCCGAAGACGTTGAAGGTGAAGCGCTGGCTACCCTGGTAGTCAACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACAGCGATGTCCTgca                                                                                                                                        <  2:378055/150‑3 (MQ=255)              gCCGAAGACGTTGAAGGTGAAGCGCTGGCTACCCTGGTAGTCAACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTc                                                                                                                           <  2:406440/150‑1 (MQ=255)               ccGAAGACGTTGAAGGTGAAGCGCTGGCTACCCTGGTAGTCAACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACAGCGATGTCCTgcag                                                                                                                                       >  1:467419/1‑134 (MQ=255)               ccGAAGACGTTGAAGGTGAAGCGCTGGCTACCCTGGTAGTCAACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACAGCGATGTCCTgcag                                                                                                                                       <  2:467419/137‑4 (MQ=255)                                           tACCCTGGTAGTCAACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGa                                                                                              >  1:559705/1‑150 (MQ=255)                                           tACCCTGGTAGTCAACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGa                                                                                              <  2:259017/150‑1 (MQ=255)                                               gtGGTAGTCAACAACATGCGCGGGATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCAATGCGGCAGGACATAGCTGTGCTGACTGGGGGCCAGGTCATATCCGAAGAAATCGGCATGTCGCTGGAAAcg                                                                                          >  2:160388/2‑149 (MQ=255)                                                                gcgcGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTCCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCt                                                                         <  1:297047/150‑1 (MQ=255)                                                                                             aGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCa                                            >  1:147012/1‑150 (MQ=255)                                                                                                 tCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGa                                        >  2:589927/1‑150 (MQ=255)                                                                                                     ggCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAac                                    <  2:209420/150‑1 (MQ=255)                                                                                                        gtCGGCGATCGCCGCAAGGCCATGCTGCAGGACATAGCTGTCATGACTGGCGGCCAGGTCATCGCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAAcacc                                 <  2:559705/149‑1 (MQ=255)                                                                                                                          gCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTACCGCCTTGCTCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCt               <  1:569888/150‑1 (MQ=255)                                                                                                                                 tgcAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTg        >  2:375116/1‑150 (MQ=255)                                                                                                                                  tcAGGACAGCGATGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGa       >  1:143709/2‑150 (MQ=255)                                                                                                                                       aCATCGCTGTCCTGACTGGAGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGAGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCg  <  2:464649/150‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                ACTGCTGATCGTTGCCGAAGACGTTGAAGGTGAAGCGCTGGCTACCCTGGTAGTCAACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCG  >  NC_002947/1550380‑1550663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑