| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,667,961 | C→T | 14.8% | A307A (GCC→GCT) | ffh → | signal recognition particle GTPase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,667,961 | 0 | C | T | 14.8% | 56.8 / 3.8 | 27 | A307A (GCC→GCT) | ffh | signal recognition particle GTPase subunit |
| Reads supporting (aligned to +/- strand): ref base C (9/14); new base T (2/2); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
GTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGC > NC_002947/1667824‑1668103 | gTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCGCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc < 1:179834/150‑1 (MQ=255) cAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaagg < 1:239599/150‑1 (MQ=255) ggCAACCCGATCAAGTTCATCGGTAGGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGCCGACGTGCTCAGGCTGAGCGAGCAGGCCGAGCAGACCATCGACaaggccaaggcc < 2:12033/150‑1 (MQ=255) aCCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACaa < 2:433019/150‑1 (MQ=255) gATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGc < 1:194901/149‑1 (MQ=255) gATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGc > 2:194901/1‑149 (MQ=255) aaGTTCATCGGTATGGGAGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGCTCCGGCAGGCCGTGGTGCACAGCGACAAGGGCGATGCCGACAAGCTgggc > 2:313562/1‑148 (MQ=255) gTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCaa < 1:403777/150‑1 (MQ=255) gtttgccggTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGCGCCCACCCAGCACACCATTGACAAAGACAACGCTTACACGCGGGCCAAGAAACt > 2:67677/8‑150 (MQ=255) ggTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGc > 2:74508/1‑140 (MQ=255) ggTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGc < 1:74508/140‑1 (MQ=255) ggTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTg < 1:259326/150‑1 (MQ=255) gaagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaa < 1:448455/141‑1 (MQ=255) gaagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaa > 2:448455/1‑141 (MQ=255) aagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaag < 2:478295/150‑1 (MQ=255) gAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTc < 2:533560/150‑1 (MQ=255) aCCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCcgcg > 1:229036/1‑150 (MQ=255) cGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCATGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGCAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCa < 1:245753/150‑1 (MQ=255) tCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGCGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATg < 1:313562/150‑1 (MQ=255) gCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTcg > 1:129630/1‑150 (MQ=255) tctGCTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTcggcg < 1:523297/147‑1 (MQ=255) tctGCTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTcggcg > 2:523297/3‑149 (MQ=255) ttggCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagg < 1:267892/72‑1 (MQ=37) ttggCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagg > 2:267892/4‑75 (MQ=37) ccTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGa > 1:474424/1‑150 (MQ=255) tGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACa > 2:205419/1‑150 (MQ=255) aGCAGGCCGGGCGGACCATCGACAAGGCCAAGGCCGACAATCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGc < 2:34803/150‑1 (MQ=255) | GTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGC > NC_002947/1667824‑1668103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |