Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,809,283 G→T 14.8% D98Y (GAC→TAC)  eno → enolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,809,2830GT14.8% 62.8 / 3.5 27D98Y (GAC→TAC) enoenolase
Reads supporting (aligned to +/- strand):  ref base G (10/13);  new base T (2/2);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

AGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTACGCGCACATCGCCAACCTGAACGGCACCCCGGGCCAGTACTCGATGCCG  >  NC_002947/1809140‑1809432
                                                                                                                                               |                                                                                                                                                     
aGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTa                                                                                                                                                 <  2:245351/150‑1 (MQ=255)
   tGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCg                                                                                                                                              <  2:331693/150‑1 (MQ=255)
                cAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCaa                                                                                                                                 <  1:20494/150‑1 (MQ=255)
                     cccGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTgg                                                                                                                            <  2:401333/149‑1 (MQ=255)
                      ccGTTACCTGGGCAAGGGCGTGCTGAAAGCCGTCGCCAACATCAACGGCCCGATCCGTGAACTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGACAACAAGGCCAAGCTtgg                                                                                                                           >  2:239072/1‑150 (MQ=255)
                       cGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGgc                                                                                                                          <  2:166631/150‑1 (MQ=255)
                                         gTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCt                                                                                                        <  2:228235/150‑1 (MQ=255)
                                         gTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCt                                                                                                        <  1:183952/150‑1 (MQ=255)
                                          tGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTg                                                                                                       >  2:392511/1‑150 (MQ=255)
                                             tGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTAcc                                                                                                                                               >  2:91889/1‑107 (MQ=255)
                                             tGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTAcc                                                                                                                                               <  1:91889/107‑1 (MQ=255)
                                                               tCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAg                                                                                  >  1:357096/1‑150 (MQ=255)
                                                               ccAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAg                                                                                  <  1:482921/149‑1 (MQ=255)
                                                                                   ctgctgctgGCCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCcaggaccagcac                                                              <  2:130990/150‑1 (MQ=255)
                                                                                         ctgGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCg                                                        >  1:54422/1‑150 (MQ=255)
                                                                                               aaGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTAc                                                  <  2:321362/150‑1 (MQ=255)
                                                                                                  gATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTAcgcg                                               <  1:392511/150‑1 (MQ=255)
                                                                                                                 aaGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTACGCGCACATCGCCAACCTg                                >  2:242795/1‑150 (MQ=255)
                                                                                                                   ggCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTACGCGCACATCGCCAACCTGaa                              >  1:557575/1‑150 (MQ=255)
                                                                                                                     ctctGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCAGGGCGCCAACGCAATCCTGGCTGTTTCCCTGCCTGCCGCCAAAGCTGCTGCCCAGGCCCAGGACCTCCCGCTGTACGCGCACATCGCCAACCTGAAca                            >  2:116454/1‑149 (MQ=255)
                                                                                                                                     gATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTACGCGCACATCGCCAACCTGAACGTCACCCCGGGCCAGTa            >  2:426844/1‑150 (MQ=255)
                                                                                                                                      aTCGAACTGTACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccaggaccagg                                                                <  2:226376/97‑1 (MQ=255)
                                                                                                                                      aTCGAACTGTACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccaggaccagg                                                                >  1:226376/1‑97 (MQ=255)
                                                                                                                                      aTCGAACTGTACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccaggaccagg                                                                <  2:529697/97‑1 (MQ=255)
                                                                                                                                      aTCGAACTGTACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccaggaccagg                                                                >  1:529697/1‑97 (MQ=255)
                                                                                                                                      aTCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCTGCTATACGCGCACATCGCAAACCTGAACGGCAACCCGGGCCAGTAc           >  2:50620/1‑150 (MQ=255)
                                                                                                                                             tGGACTGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTACGCGCACATCGCCAACCTGATCGGCACCCCGGGCCAGTACTCGATGc    <  1:487054/150‑1 (MQ=255)
                                                                                                                                               gACGGTACGGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTACGTGCACATCGCCAACCTGAACGGCACCCCGGGCCAGTACTCGATGCCg  <  2:112350/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
AGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGGACCAGGACCTGCCGCTGTACGCGCACATCGCCAACCTGAACGGCACCCCGGGCCAGTACTCGATGCCG  >  NC_002947/1809140‑1809432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑