| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 170,295 | C→A | 21.1% | R233S (CGT→AGT) | PP_0159 → | family III CoA‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 170,295 | 0 | C | A | 21.1% | 31.5 / 4.6 | 19 | R233S (CGT→AGT) | PP_0159 | family III CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base C (9/6); new base A (2/2); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01 | |||||||||||
GGTTGTATTCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAG > NC_002947/170146‑170435 | ttctGTATTCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTcccc < 2:596881/147‑1 (MQ=255) tATTCCGCGGTCGCCATGCTGGCTGCCCTCGAACATCGTGATCATACAGGGGGCGGCCCCGCTGTCGACATGGCGTCGATCCATTCGC‑GCTCGGGCACGT‑TGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCgagat > 1:596881/1‑146 (MQ=255) ccATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATgcgc > 1:244074/1‑150 (MQ=255) ccATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATgcgc < 2:491715/150‑1 (MQ=255) tCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTcc > 1:520777/1‑150 (MQ=255) gCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCAGTCgg > 1:80394/1‑120 (MQ=255) gCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCAGTCgg < 2:80394/120‑1 (MQ=255) gCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCAGTCgg < 2:80377/120‑1 (MQ=255) gCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCAGTCgg > 1:80377/1‑120 (MQ=255) ccAGCATGTCGACATGGCATTGCTTGATGTGGAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATgg < 2:40419/150‑1 (MQ=255) gACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCAtt > 2:141812/1‑150 (MQ=255) gCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCCCCCCACTACGTCTGTCAGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCgg < 1:380732/150‑1 (MQ=255) cAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGAAAGc > 1:196386/1‑150 (MQ=255) gCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACGAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTAGGTAATGACAGCCAGTTCCGCAAGTTc > 2:223939/1‑150 (MQ=255) cGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCg < 1:293015/150‑1 (MQ=255) aGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATgc < 1:59009/52‑1 (MQ=255) aGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATgc > 2:59009/1‑52 (MQ=255) gAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCgg > 1:4455/1‑150 (MQ=255) gCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCgg > 2:484989/1‑150 (MQ=255) aCCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGTCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCggag < 1:18441/150‑1 (MQ=255) | GGTTGTATTCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGT‑‑GGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAG > NC_002947/170146‑170435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |