| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,859,504 | A→T | 14.3% | intergenic (‑54/+102) | PP_1662 ← / ← PP_1663 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,859,504 | 0 | A | T | 14.3% | 68.3 / 5.6 | 28 | intergenic (‑54/+102) | PP_1662/PP_1663 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (16/8); new base T (2/2); total (18/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
CCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGT > NC_002947/1859356‑1859638 | ccTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACaa < 2:581938/150‑1 (MQ=255) gggACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCAt > 1:273978/1‑150 (MQ=255) gggACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCAt > 1:97832/1‑150 (MQ=255) cagagtcgtcgCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGa > 1:21716/6‑150 (MQ=255) ttGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCt > 1:151408/1‑150 (MQ=255) gTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACTTGTTTCAt < 2:123717/105‑1 (MQ=255) gTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACTTGTTTCAt > 1:123717/1‑105 (MQ=255) tAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCtgggtgg > 2:96023/1‑132 (MQ=255) tAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCtgggtgg < 1:96023/132‑1 (MQ=255) cTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCt < 1:327613/122‑1 (MQ=255) cTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCt > 2:327613/1‑122 (MQ=255) tCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGc > 1:179450/1‑150 (MQ=255) aaGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTggg > 1:218118/1‑150 (MQ=255) aTGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCgg < 2:273978/150‑1 (MQ=255) cGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCa < 2:97832/150‑1 (MQ=255) cGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCTTAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgcagg < 2:21716/150‑1 (MQ=255) tcttctTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTgc < 1:351926/150‑1 (MQ=255) cttcttTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTgcg > 2:365644/1‑150 (MQ=255) cttTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCGGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGa < 2:151408/150‑1 (MQ=255) agTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCgggg < 1:309667/116‑1 (MQ=255) agTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCgggg > 2:309667/1‑116 (MQ=255) agTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGc > 1:276320/1‑150 (MQ=255) agTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTGTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCATGTATTGCAGGCAGTTCGCGATCAGTTga > 2:129976/1‑149 (MQ=255) ttAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCa > 1:430438/1‑150 (MQ=255) gCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCg > 2:371154/1‑150 (MQ=255) taagtaagTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGc > 2:602965/1‑150 (MQ=255) aaaCAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGt > 2:302502/1‑150 (MQ=255) cttcATGAAACTTGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgcag > 2:344044/4‑102 (MQ=255) cttcATGAAACTTGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgcag < 1:344044/99‑1 (MQ=255) | CCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGT > NC_002947/1859356‑1859638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |