| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,166,687 | T→A | 15.6% | intergenic (‑488/‑293) | PP_5483 ← / → PP_1921 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,166,687 | 0 | T | A | 15.6% | 77.4 / 6.4 | 32 | intergenic (‑488/‑293) | PP_5483/PP_1921 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (12/15); new base A (3/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
TTTTTTCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGA > NC_002947/2166542‑2166826 | ttttttCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAg > 1:176277/1‑150 (MQ=255) ttttCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCg < 2:486383/150‑1 (MQ=255) ccGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGgatga > 2:198850/1‑150 (MQ=255) gCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAggg < 1:558710/150‑1 (MQ=255) aTTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCt < 2:150820/150‑1 (MQ=255) aGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCAGGAGCgggt < 2:553908/133‑2 (MQ=255) aGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCAGGAGCgggt > 1:553908/1‑132 (MQ=255) ggCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTg < 1:198850/150‑1 (MQ=255) gTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAg < 1:76755/150‑1 (MQ=255) cGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCtcaatcaa > 1:17421/1‑150 (MQ=255) gTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAAc > 1:271210/1‑150 (MQ=255) ccTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTc > 2:415243/1‑150 (MQ=255) tCAGAAAGTTACTCCCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGtt < 1:221049/150‑1 (MQ=255) aaGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACTACCCGCTCCTGGAGCCGAATGATCCTAGCCCTGTTGGGGTTTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGCTTACAgc < 2:57675/150‑2 (MQ=255) tctcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagg < 1:415243/150‑1 (MQ=255) tATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagggagtagggagg > 1:384449/1‑149 (MQ=255) tATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagggagtag < 1:212910/145‑1 (MQ=255) tATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagggagtag < 1:212875/145‑1 (MQ=255) tATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagggagtag > 2:212910/1‑145 (MQ=255) tATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagggagtag > 2:212875/1‑145 (MQ=255) ttGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagggagtagggagtag < 2:100690/150‑1 (MQ=255) gTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGACCTGCCGGTGCGGAGGGCTGTGGGGGTGTGCCTGGTTGAGTGAAGC‑‑‑ATATACCTGGCTGTTCCAGGTTGCAGGTCgtagggagtagggagtagggagtaggga < 1:417784/150‑1 (MQ=255) gCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTg < 2:128939/70‑1 (MQ=255) gCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTg > 1:128939/1‑70 (MQ=255) ccATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTGGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTGGGGAGTAGGGAGTAGGGGCAGGGGGAGTc > 1:130097/1‑150 (MQ=255) gtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCgg < 2:17421/150‑1 (MQ=255) gtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCgg > 2:144746/1‑150 (MQ=255) gAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagggagtag < 1:302982/106‑1 (MQ=255) gAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtagggagtag > 2:302982/1‑106 (MQ=255) atcccGCTCCAGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGa > 2:258334/3‑150 (MQ=255) tcccGCTCCAGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtag > 2:165108/2‑93 (MQ=255) tcccGCTCCAGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCgtag < 1:165108/92‑1 (MQ=255) | TTTTTTCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGC‑‑TTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGA > NC_002947/2166542‑2166826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |