| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,208,180 | A→T | 13.9% | N117Y (AAT→TAT) | PP_1951 → | short‑chain dehydrogenase/reductase family oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,208,180 | 0 | A | T | 13.9% | 93.6 / 8.7 | 36 | N117Y (AAT→TAT) | PP_1951 | short‑chain dehydrogenase/reductase family oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (11/20); new base T (3/2); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01 | |||||||||||
TGTGACGGACCGAGATGCTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAACCGATCTGGCTCAATTATTGTGACCACCTCGGTGTCGG > NC_002947/2208035‑2208324 | tgtgACGGACCGAGATGCTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAatat < 2:579240/150‑1 (MQ=255) gACGGACCGAGATGCTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCt > 1:234586/1‑150 (MQ=255) gACGGACCGAGATGCTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCt < 2:299597/150‑1 (MQ=255) cGAGATGCTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCATATTTGCTggttctc < 1:155761/150‑6 (MQ=255) tGCTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTc < 2:435130/150‑1 (MQ=255) tGCTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTc < 2:181283/150‑1 (MQ=255) cTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCgg > 2:93560/1‑150 (MQ=255) aaTGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAg < 2:528379/150‑1 (MQ=255) aaTGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAg < 2:230291/150‑1 (MQ=255) aaTGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAg < 2:234586/150‑1 (MQ=255) cTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTg < 1:491146/116‑1 (MQ=255) cTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTg > 2:491146/1‑116 (MQ=255) tGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCAt > 2:410489/1‑150 (MQ=255) agcagGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTgga > 1:283310/1‑134 (MQ=255) agcagGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTgga < 2:283310/134‑1 (MQ=255) agcagGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGAGCCAGGACCTTGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGt < 1:23249/150‑1 (MQ=255) aTTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCtgt < 1:588295/150‑1 (MQ=255) ggACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGgaa > 2:70676/1‑94 (MQ=255) ggACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGgaa < 1:70676/94‑1 (MQ=255) ggACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGtt < 1:93560/150‑1 (MQ=255) gACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCATATTTGCTggttc < 2:263353/94‑4 (MQ=255) gACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCATATTTGCTggttc > 1:263353/1‑91 (MQ=255) tgtTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGa < 1:297096/150‑1 (MQ=255) gtTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGAt > 2:479758/1‑150 (MQ=255) gtTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGAt < 1:428082/150‑1 (MQ=255) tCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACAt > 1:532508/1‑150 (MQ=255) tCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACAt > 1:509685/1‑150 (MQ=255) gACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGagag < 1:571741/150‑1 (MQ=255) ggATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAAc < 2:532508/150‑1 (MQ=255) gCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAACCGATCt > 1:506961/1‑150 (MQ=255) cGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAACCGATCTGGCTCAATTATtgt < 1:410489/150‑1 (MQ=255) gggAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAACCGATCTGGCTCAATTATTGTGacca < 2:506961/150‑1 (MQ=255) aaCGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAACCGATCTGGCTCAATTATTGTGACCACCt > 1:259749/1‑150 (MQ=255) tcttccAGCATATTTGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGCGAGCTAACCGATCTGGCTCAATTATTGTGACCACCTCGgt > 2:49509/5‑150 (MQ=255) ttccAGCATATTTGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAACCGATCTGGCTCAATTATTGTGACCACCTCGgtgt > 1:247569/3‑150 (MQ=255) cAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAACCGATCTGGCTCAATTATTGTGACCACCTCGGTGTCgg < 2:509685/150‑1 (MQ=255) | TGTGACGGACCGAGATGCTGTAGATGATACGTTTAATGCTGTAGCAGCAGGCTTTGGTGGATTGGACATTGTGTTCGCCAACGCCGGGATTGATCCAGGACCTGGATTCGCTGCACTCAATGCCGCAGGGGAACGAGAACCAGCAAATATGCTGGAAGAATACTCGGACCATCGCTGGAGGAAGGTCATCTCCGTCAGCCTAGATGCTGTGTTCTATTCGATAAGAGCTGCTGCGCGGCACATGAGAGCTAACCGATCTGGCTCAATTATTGTGACCACCTCGGTGTCGG > NC_002947/2208035‑2208324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |