| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,517,645 | T→C | 14.3% | S8P (TCC→CCC) | PP_2211 → | AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,517,645 | 0 | T | C | 14.3% | 62.0 / 4.0 | 28 | S8P (TCC→CCC) | PP_2211 | AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (13/11); new base C (2/2); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
TGCGCCTGATTCAGGCATTCCTGAGCCTGGCAGCAGGGCTATGATGGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCAAGGGTCAGTGCCGAGTCCTATGCCCGCCTGTG > NC_002947/2517503‑2517790 | tGCGCCTGATTCAGGCATTCCTGAGCCTGGCAGCAGGGCTATGATGGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCa > 1:22533/1‑150 (MQ=255) aTTCAGGCATTCCTGAGCCTGGCAGCAGGGCTATGATGGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTgc > 1:97859/1‑150 (MQ=255) ggCATTCCTGAGCCTGGCAGCAGGGCTATGATGGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGaa > 1:364129/1‑150 (MQ=255) ggCATTCCTGAGCCTGGCAGCAGGGCTATGATGGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTCCCATGCAGCTGGTGCGCGaa > 1:414295/1‑150 (MQ=255) cATTCCTGAGCCTGGCAGCAGGGCTATGATGGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAgc < 1:269018/150‑1 (MQ=255) ttCCTGAGCTTGGCAGCAGGGCTATGATGGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAgcgc < 2:572219/150‑1 (MQ=255) gatgGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGa < 2:292151/150‑1 (MQ=255) tttGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATg < 1:483775/98‑1 (MQ=255) tttGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATg > 2:483775/1‑98 (MQ=255) aCCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGAcctgccctgc < 1:179676/123‑1 (MQ=255) aCCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGAcctgccctgc > 2:179676/1‑123 (MQ=255) tCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAgcgc < 1:223327/100‑1 (MQ=255) tCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAgcgc > 2:223327/1‑100 (MQ=255) ggCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAgggc < 2:364129/150‑1 (MQ=255) cTTTTCATACAGACCCTAATATGGTCGGCAATCAGCCGACAGGACCGTCGATGAGCAACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGACTGACAGTGGCTTGCTGGCTAgggcgg > 1:591240/1‑150 (MQ=255) ccTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGaa > 2:446642/1‑150 (MQ=255) cTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTCCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGaac < 2:414295/150‑1 (MQ=255) ccGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGc > 2:99859/1‑110 (MQ=255) ccGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGc < 1:99859/110‑1 (MQ=255) tctaaTCAGCCGCAAGGCACCTCGATGAGCGACAAAGACACCATTCCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTg < 2:142732/147‑1 (MQ=255) aTCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTCCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGcaga > 1:142732/1‑147 (MQ=255) ccGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCa > 2:522198/1‑150 (MQ=255) gACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCAAg > 1:51387/1‑150 (MQ=255) aCAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCAAgg < 2:22533/150‑1 (MQ=255) gATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCAAGGGTCAGTGCCGa < 2:97859/150‑1 (MQ=255) aGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCAAGGGTCAGTGCCGAGTCCTATGCCCg > 1:516651/1‑150 (MQ=255) aGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCAAGGGTCAGTGCCGAGTCCTATGCCCg > 1:447786/1‑150 (MQ=255) cATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCAAGGGTCAGTGCCGAGTCCTATGCCCGCCtgtg < 1:496162/150‑1 (MQ=255) | TGCGCCTGATTCAGGCATTCCTGAGCCTGGCAGCAGGGCTATGATGGCTGTTTGCTACGGGGCACCCTCGGCTTTTCATACAGACCCTAATATGGCCGGCAATCAGCCGACAGGACCGTCGATGAGCGACAAAGACACCATTTCCATGCAGCTGGTGCGCGAAGCGCTGTTGCAGACCTGCCCTGCCGGTGAGCCTGACAGTGGCTTGCTGGCCAGGGCGGGCATCGCCGTTGAACAACTGCACCTGCCAGAGGCAAGGGTCAGTGCCGAGTCCTATGCCCGCCTGTG > NC_002947/2517503‑2517790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |