Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,563,408 A→G 13.3% E368G (GAG→GGG)  dauA → FAD‑dependent catabolic D‑arginine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,563,4080AG13.3% 68.9 / 4.0 30E368G (GAG→GGG) dauAFAD‑dependent catabolic D‑arginine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (13/13);  new base G (2/2);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

TCGATGCGCATGCCCCCGCGTTTTTCTGGCTGGCGGCCCAGGGGGGCTATGGCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAGGCCGGTGGCTTTCTGT  >  NC_002947/2563261‑2563533
                                                                                                                                                   |                                                                                                                             
tCGATGCGCATGCCCCCGCGTTTTTCTGGCTGGCGGCCCAGGGGGGCTATGGCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAgc                                                                                                                             <  2:237448/150‑1 (MQ=255)
                     ttttCTGGCTGGCGGCCCAGGGGGGCTATGGCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCTCGGGCGccacg                                                                                                                       >  2:597410/1‑132 (MQ=255)
                     ttttCTGGCTGGCGGCCCAGGGGGGCTATGGCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCTCGGGCGccacg                                                                                                                       <  1:597410/135‑4 (MQ=255)
                            gctggcGGCCCAGGGGGGCTATGGCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGTCAGCCTTGTCAGCCAGGGCGTGGCGCTCGGGCGcc                                                                                                                          >  2:505100/1‑125 (MQ=255)
                            gctggcGGCCCAGGGGGGCTATGGCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGTCAGCCTTGTCAGCCAGGGCGTGGCGCTCGGGCGcc                                                                                                                          <  1:505100/125‑1 (MQ=255)
                                   gcCCAGGGGGGCTATGGCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAg                                                                                          >  1:285058/1‑150 (MQ=255)
                                                  ggCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCAGTCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGc                                                                           >  1:491467/1‑150 (MQ=255)
                                                     aTCCGGGCGGCAGCTTGCGCTGCTCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAAt                                                                        <  2:285058/150‑1 (MQ=255)
                                                                    ggCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTc                                                         <  2:491467/150‑1 (MQ=255)
                                                                        ccTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCca                                                     <  2:176717/150‑1 (MQ=255)
                                                                               ccTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTTTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCt                                              >  2:50152/1‑150 (MQ=255)
                                                                                          acgtGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGt                                   <  1:215436/147‑1 (MQ=255)
                                                                                            cTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGaaa                                                                                           <  1:34518/92‑1 (MQ=255)
                                                                                            cTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGaaa                                                                                           >  2:34518/1‑92 (MQ=255)
                                                                                              gCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTc                               >  1:416900/1‑150 (MQ=255)
                                                                                                   tGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCcgcg                          >  1:281902/1‑150 (MQ=255)
                                                                                                    gggCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCcgcgc                         <  2:171581/150‑1 (MQ=255)
                                                                                                            cGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGACCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAgg                 >  2:208812/1‑150 (MQ=255)
                                                                                                                  gtgCCAGCCTTTTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAGGCCGGTg           <  1:50152/150‑1 (MQ=255)
                                                                                                                    gCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAGGCCGGTGGc         >  1:363918/1‑150 (MQ=255)
                                                                                                                     ccAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATccc                                                                                 <  2:15519/77‑1 (MQ=255)
                                                                                                                     ccAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATccc                                                                                 >  1:15519/1‑77 (MQ=255)
                                                                                                                           ttGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAGGCCGGTGGCTTTCTGt  >  2:570705/1‑150 (MQ=255)
                                                                                                                           ttGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAGGCCGGTGGCTTTCTGt  <  2:584381/150‑1 (MQ=255)
                                                                                                                                  ccAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAGGCCGGTGGCt        >  2:16752/1‑137 (MQ=255)
                                                                                                                                  ccAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAGGCCGGTGGCt        <  1:16752/137‑1 (MQ=255)
                                                                                                                                    aGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGc                                                              <  2:43368/81‑1 (MQ=255)
                                                                                                                                    aGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGc                                                              >  1:43368/1‑81 (MQ=255)
                                                                                                                                            gcgcCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGt                                   <  2:507369/100‑1 (MQ=255)
                                                                                                                                            gcgcCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGt                                   >  1:507369/1‑100 (MQ=255)
                                                                                                                                                   |                                                                                                                             
TCGATGCGCATGCCCCCGCGTTTTTCTGGCTGGCGGCCCAGGGGGGCTATGGCATCCAGTCGGCAGCGGGCGCCTCGCGCCTGGCCGCCGACCTGCTACTGGGCCAGCCGTTGTGTGCCAGCCTTGTCAGCCAGGGCGTGGCGCCCGAGCGCCTGTCCCCGGCCCGTTTCCAGCAACCCTGAAAGGAGCATCCCATGAGCAATGACATTCAGCGTTTCCCCAGCAGCCTGCCGTTCCCGTTTTCCCGCGCGGTAAAGGCCGGTGGCTTTCTGT  >  NC_002947/2563261‑2563533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑