| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,654,922 | G→A | 18.2% | A190T (GCC→ACC) | cysB → | transcriptional regulator CysB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,654,922 | 0 | G | A | 18.2% | 41.5 / 4.8 | 22 | A190T (GCC→ACC) | cysB | transcriptional regulator CysB |
| Reads supporting (aligned to +/- strand): ref base G (10/8); new base A (2/2); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
TTCGCCATCGCCACCGAGGCGCTGGAGCTGTTCGGCGACCTGATCATGATGCCGTGCTACAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGACGTATGTGCGCCTGGGGTTGG > NC_002947/2654787‑2655069 | ttCGCCATCGCCACCGAGGCGCTGGAGCTGTTCGGCGACCTGATCATGATGCCGTGCTACAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATc < 2:254788/150‑1 (MQ=255) gccaCCGAGGCGCTGGAGCTGTTCGGCGACCTGATCATGATGCCGTGCTACAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTAc < 1:306870/150‑1 (MQ=255) aTCATGATGCCGTGCTACAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGAt > 2:57760/1‑150 (MQ=255) tgatgCCGTGCTACAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCg < 2:297068/120‑1 (MQ=255) tgatgCCGTGCTACAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCg > 1:297068/1‑120 (MQ=255) gCCGTGCTACAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCttt < 2:121430/150‑1 (MQ=255) cAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCg > 1:420773/1‑150 (MQ=255) tGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTg > 1:470997/1‑126 (MQ=255) tGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTg < 2:470997/126‑1 (MQ=255) cgttgcgttgTAGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACg > 2:152894/1‑150 (MQ=255) ttgcgttgTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGcc > 1:192782/1‑150 (MQ=255) ggtgCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGt < 1:29856/150‑1 (MQ=255) gggCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACcgcg > 1:348174/1‑150 (MQ=255) gCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGc > 1:485315/1‑150 (MQ=255) gAAACTGACCCTGGAAGCCGTCACCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGAc > 2:223891/1‑150 (MQ=255) gAAACTGACCCTGGAAGCCGTCACCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCGCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGAc > 2:224216/1‑150 (MQ=255) cTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGACGTAt > 1:486960/1‑150 (MQ=255) gAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGACGTATGTGCGCCTg > 2:379479/1‑150 (MQ=255) aaGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGACGTATGTGCGCCTgg < 2:351232/150‑1 (MQ=255) ccGTCACCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGACGTATGTGCGCCTGGGGt < 1:224216/150‑1 (MQ=255) ccGTCACCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGACGTATGTGCGCCTGGGGt < 1:223891/150‑1 (MQ=255) tCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGACGTATGTGCGCCTGGGGTTgg < 2:486960/150‑1 (MQ=255) | TTCGCCATCGCCACCGAGGCGCTGGAGCTGTTCGGCGACCTGATCATGATGCCGTGCTACAAGTGGAACCGTTGCGTTGTGGTGCCGCAGGGGCACCCGCTGGCCAAGCTGCCGAAACTGACCCTGGAAGCCGTCGCCGAGTACCCGATCGTCACCTACGTGTTCGGTTTCACCGGGCGCTCAAAGCTGGATGAAGCTTTCAATCACCGTGGCCTCACGCCAAAAGTGGTCTTCACCGCGGCTGACGCCGACGTGATCAAGACGTATGTGCGCCTGGGGTTGG > NC_002947/2654787‑2655069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |