| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,868,920 | G→C | 16.6% | intergenic (+416/‑515) | PP_2520 → / → PP_2522 | hypothetical protein/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,868,920 | 0 | G | C | 16.6% | 56.3 / 6.6 | 26 | intergenic (+416/‑515) | PP_2520/PP_2522 | hypothetical protein/transposase |
| Reads supporting (aligned to +/- strand): ref base G (8/12); new base C (2/2); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
AGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGC > NC_002947/2868781‑2869059 | aGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGt > 2:338300/1‑149 (MQ=255)aGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGt < 1:338300/149‑1 (MQ=255) cTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATc < 2:45922/150‑1 (MQ=255) cattggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatcaa > 2:410490/1‑150 (MQ=255) ccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTc < 1:322777/150‑1 (MQ=255) ccATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGccc > 1:428363/1‑150 (MQ=255) aTGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGtt > 2:59820/1‑136 (MQ=255) aTGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGtt < 1:59820/136‑1 (MQ=255) ccTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTc < 2:428363/150‑1 (MQ=255) ggggCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACg < 2:383532/150‑1 (MQ=255) gggCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGAtt < 1:34778/139‑1 (MQ=255) gggCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGAtt > 2:34778/1‑139 (MQ=255) cAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGCCCGACGGCtt > 2:479886/1‑150 (MQ=255) tCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGt > 1:267015/1‑128 (MQ=255) tCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGt < 2:267015/128‑1 (MQ=255) tGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTcaca < 2:454063/150‑1 (MQ=255) cTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGTGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAg > 1:202273/1‑130 (MQ=255) cTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGTGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAg < 2:202273/130‑1 (MQ=255) gAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCACCAATGCCCCGGCATTg < 1:560728/51‑1 (MQ=25) gAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCACCAATGCCCCGGCATTg > 2:560728/1‑51 (MQ=25) ttCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATAcc < 1:410490/150‑1 (MQ=255) gggcggCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCt < 1:216991/150‑1 (MQ=255) gcCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGCCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTc < 1:479886/150‑1 (MQ=255) atcaccaATGCCCCGGCATTGATGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGttt > 2:80213/6‑150 (MQ=255) tcaccaATGCCCCGGCATTGATGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTc < 1:80213/150‑1 (MQ=255) atcaATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTcgc > 1:144101/1‑150 (MQ=255) | AGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGC > NC_002947/2868781‑2869059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |