| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,020,315 | G→C | 12.9% | W270C (TGG→TGC) | PP_2638 → | cellulose synthase operon protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,020,315 | 0 | G | C | 12.9% | 78.5 / 6.2 | 31 | W270C (TGG→TGC) | PP_2638 | cellulose synthase operon protein C |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base C (2/2); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
TCCGGGCAAGCCGTGAGTGCCACCAGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCATGGGGCCCTGGGTTATGCGTTGATGCGCCAGGGCC > NC_002947/3020181‑3020457 | tCCGGGCAAGCCGTGAGTGCCACCAGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACgg > 1:53299/1‑150 (MQ=255) ccGGGCAAGCCGTTACGGCCACCAGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCCGCATGGCAGGCCGGTCAACcgg < 1:212339/150‑1 (MQ=255) gCAAGCCGTGAGTGCCACCAGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACCTGCCGGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTaa > 1:412606/1‑150 (MQ=255) aaGCCGTGAGTGCCACCAGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAgg < 1:121375/150‑1 (MQ=255) cGTGATTGCCACCAGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCAtt < 1:501370/150‑1 (MQ=255) gAGTGCCACCAGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCt < 1:371894/150‑1 (MQ=255) accaGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAg > 1:351984/1‑150 (MQ=255) caGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACggg < 2:41237/128‑1 (MQ=255) caGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACggg > 1:41237/1‑128 (MQ=255) gcgGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCCTGCCATGCCgggtc > 2:434743/1‑116 (MQ=255) gcgGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCCTGCCATGCCgggtc > 2:434712/1‑116 (MQ=255) gcgGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCCTGCCATGCCgggtc < 1:434712/119‑4 (MQ=255) gcgGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCCTGCCATGCCgggtc < 1:434743/119‑4 (MQ=255) aaGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGa < 2:47240/145‑1 (MQ=255) aaGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGa > 1:47240/1‑145 (MQ=255) ttcttcAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGc > 2:48747/1‑150 (MQ=255) tcttcAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCa < 1:134757/150‑1 (MQ=255) aGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTgc > 1:249132/1‑150 (MQ=255) cGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTgcgc > 1:519342/1‑150 (MQ=255) ccGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCa > 1:69875/1‑150 (MQ=255) gCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCa < 1:309217/150‑1 (MQ=255) gCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCa > 1:289355/1‑150 (MQ=255) tGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCgg < 1:239766/150‑1 (MQ=255) caGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGAt < 2:289355/150‑1 (MQ=255) gaCCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAAAGGGGTAAGGCATTGCAAGCAAAGGGCTTTTATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCa > 1:380989/1‑150 (MQ=255) cTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCATgg < 2:519342/150‑1 (MQ=255) cAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCATGGGGcc > 1:219764/1‑150 (MQ=255) agcagcGCAAGCTGCTCGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAACCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCATGGGGCCCTgg < 1:230801/150‑1 (MQ=255) gctgctGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAATCGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCATGGGGCCCTGGGTTATGCGtt < 2:292445/150‑1 (MQ=255) gCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCATGGGGCCCTGGGTTATGCGTTGATgcgc < 2:249132/150‑1 (MQ=255) cGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCATGGGGCCCTGGGTTATGCGTTGATGCGCCAGGgcc < 1:203039/150‑1 (MQ=255) | TCCGGGCAAGCCGTGAGTGCCACCAGCGCGGCAGCCTGGCAAGCCTTTCTTCAGCGTTACCCGGCTTCGCCGTTGCTGGCACAGGCCAGCGAGACCTTGCAACAGCAGCGCAAGCTGCTGGCCGACCCGGCATGGCAGGCCGGTCAACGGGGTAAGGCATTGCTCGACAAGGGCCTTAATGCCGAAGCGGAAACGCAGCTGCGCCGCGCACTGCGCCAGTACCCGGGCGATGCCAGCCTGCATGGGGCCCTGGGTTATGCGTTGATGCGCCAGGGCC > NC_002947/3020181‑3020457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |