| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,313,868 | C→A | 20.1% | intergenic (+30/‑129) | hemB → / → proP | 5‑aminolevulinate dehydratase/osmosensory proline/betaine/H+ permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,313,868 | 0 | C | A | 20.1% | 34.3 / 4.0 | 20 | intergenic (+30/‑129) | hemB/proP | 5‑aminolevulinate dehydratase/osmosensory proline/betaine/H+ permease |
| Reads supporting (aligned to +/- strand): ref base C (7/9); new base A (2/2); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCAAGA > NC_002947/3313734‑3314012 | gacgaAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATAAAAGCCGCTGCAATGGCAGCGGCtttc < 2:127509/150‑2 (MQ=255) aGGCCGGGTGGTGCTCGAAATCATTTGCGCAAGCAAGCGTGGTGGTGCAGACCTGATCCTGACCTATTTCGCTATGGACCTGGCGCGCGAAGTTATGTGAGGCCAGGCCTTGGTCGTGAGAGCCGCTGCCATTGCAGCGGCTTTTCTTtg < 2:85780/150‑1 (MQ=255) gtggtgCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCgg > 2:567173/1‑133 (MQ=255) gtggtgCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCgg < 1:567173/133‑1 (MQ=255) tGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCaaa < 2:384756/150‑1 (MQ=255) gTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACa < 2:524890/150‑1 (MQ=255) aCTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGc > 1:34348/1‑73 (MQ=255) aCTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGc < 2:34348/73‑1 (MQ=255) cTGGCGCGCGAAGGTATCTGACGCCAGGCCTTGGGCATTAGAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATccc > 1:14101/1‑150 (MQ=255) cTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATccc > 2:368166/1‑150 (MQ=255) cgcgAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTagag < 1:295851/150‑1 (MQ=255) aTCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCg > 1:579683/1‑150 (MQ=255) tGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACa < 2:579683/150‑1 (MQ=255) gCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACa > 2:267475/1‑82 (MQ=255) gCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACa < 1:267475/82‑1 (MQ=255) tCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGaa < 1:368166/150‑1 (MQ=255) aaGCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCg > 1:313131/1‑150 (MQ=255) gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATc > 2:178345/1‑101 (MQ=255) gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATc < 1:178345/101‑1 (MQ=255) gCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCAAGa > 2:548840/1‑150 (MQ=255) ccATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCt < 2:402821/102‑1 (MQ=255) ccATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCt > 1:402821/1‑102 (MQ=255) | GACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCAAGA > NC_002947/3313734‑3314012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |