| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,345,870 | C→T | 11.4% | A225T (GCC→ACC) | PP_2942 ← | two‑component system response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,345,870 | 0 | C | T | 11.4% | 84.0 / 2.9 | 35 | A225T (GCC→ACC) | PP_2942 | two‑component system response regulator |
| Reads supporting (aligned to +/- strand): ref base C (15/16); new base T (2/2); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
TGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCTGTGTTTCAGCTCTTCGTAGGCCA > NC_002947/3345722‑3346007 | tGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCg < 2:309396/150‑1 (MQ=255) ggCGTGGGTAGTTGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGt < 1:398954/150‑1 (MQ=255) ggCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGt < 2:5168/150‑1 (MQ=255) ggCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGt < 2:330000/150‑1 (MQ=255) ggCGTGGGTAGTCGCGATAGCGGTCGAGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGt < 2:222856/150‑1 (MQ=255) ggTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAGCGATCATGCTATCCGCCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCAAGATCGCGGCTGATCGCCGCGTCGAAGGCGTGGAccc > 2:43947/1‑150 (MQ=255) gTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACa < 2:206472/150‑1 (MQ=255) gtcgcTATGCATCCACTGGTCCGAAGGGGCAACGATCATGCTCTACGG‑CAACCTACCAAACATAGTACTGAACAGCGTCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTc < 1:43947/150‑1 (MQ=255) gcTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGCAGCAACGCACCAGTTCgat < 2:513664/150‑1 (MQ=255) gtgCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGc > 1:94635/1‑136 (MQ=255) gtgCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGc < 2:94635/136‑1 (MQ=255) tCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGACTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATATGCTGATTGGTc > 2:284968/1‑150 (MQ=255) ccGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTc < 2:451114/141‑1 (MQ=255) ccGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTc > 1:451114/1‑141 (MQ=255) gATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAgg > 2:130583/1‑150 (MQ=255) cATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAg < 2:229130/150‑1 (MQ=255) tGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCCACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGcc > 1:17583/1‑150 (MQ=255) aTCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAg > 2:472514/1‑150 (MQ=255) aaCTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGTGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCa > 1:386158/1‑150 (MQ=255) aaCTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCa > 2:164090/1‑150 (MQ=255) aaTTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGGGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATc > 1:447796/1‑150 (MQ=255) gCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGaa < 1:472514/150‑1 (MQ=255) cTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGTGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAaca < 2:386158/150‑1 (MQ=255) cTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGa < 1:83009/101‑1 (MQ=255) cTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGa > 2:83009/1‑101 (MQ=255) tA‑TTTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGTGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAacac > 2:380174/1‑150 (MQ=255) ccATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGc > 1:19535/1‑150 (MQ=255) aGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCTGTGTTTc < 2:19535/150‑1 (MQ=255) tCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCTGTGTTTCAGc > 2:131004/1‑150 (MQ=255) ggCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCt > 2:245886/1‑136 (MQ=255) ggCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCt < 1:245886/136‑1 (MQ=255) gCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCTGTGTTTCAGCTCTTc < 1:130583/150‑1 (MQ=255) gATCGCCTCGTCCAAGGTGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCTGTGTTTCAGCTCTTCGTa < 1:380174/150‑1 (MQ=255) tCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGGTTGGACTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCTGTGTTTCAGCTCTTCGTAgg > 1:168340/1‑150 (MQ=255) ccTCGTCCAAGGCGTGGACCCTACAGTAGAAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCTGTGTTTCAGCTCTTCGTAGGCCa > 1:239822/1‑150 (MQ=255) | TGGCGTGGGTAGTCGCGATAGCGGTCGCGGTCGCTGTGCATCAACTGGTCCGAAGGGGCAACGATCATGCTATCCGGCCAACTCAATTTGCCTA‑TGTTGTACAGCGCCGCAGCCATGGCCAGATCGCGGCTGATCGCCTCGTCCAAGGCGTGGACCCTACAGTAGCAACGCACCAGTTCGATGATCTGTCGATTGGTCTGCTTGGCTGGAGGCAGCCGCAGATTAGCCAACATCGAGAACACCTCGGTCCCGGTCACATAGCTGTGTTTCAGCTCTTCGTAGGCCA > NC_002947/3345722‑3346007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |