Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,816,546	A→T	16.0%	G106G (GGT→GGA)	cpxR ←	two‑component system regulator CpxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,816,546	0	A	T	16.0%	61.2 / 5.2	25	G106G (GGT→GGA)	cpxR	two‑component system regulator CpxR
Reads supporting (aligned to +/- strand):  ref base A (10/11);  new base T (2/2);  total (12/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGGA  >  NC_002947/3816402‑3816689                                                                                                                                                 |                                                                                                                                                tGCGCCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCggg                                                                                                                                            >  1:220976/1‑150 (MQ=255)    gcCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCTCCCGgtga                                                                                                                                         >  1:246199/1‑150 (MQ=255)        cAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTg                                                                                                                                     >  2:202327/1‑150 (MQ=255)           cgcgcCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCTCCCGGTGAGCTggtg                                                                                                                                  <  1:137035/150‑3 (MQ=255)           cgcgcCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCTCCCGGTGAGCTggtg                                                                                                                                  <  2:246199/150‑3 (MQ=255)            gcgcCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGGTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCt                                                                                                                                 >  1:501866/1‑150 (MQ=255)              gcCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTg                                                                                                                               <  1:154423/150‑1 (MQ=255)                           ccGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCAt                                                                                                                  >  1:598191/1‑150 (MQ=255)                               ccACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGc                                                                                                              <  2:493228/150‑1 (MQ=255)                                 acaTTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGccc                                                                                                            <  2:598191/150‑1 (MQ=255)                                               ccACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTCTTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCg                                                                                              <  1:41046/150‑1 (MQ=255)                                                                                cTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTc                                                             <  2:13352/150‑1 (MQ=255)                                                                                   gTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTcagc                                                          >  1:166164/1‑150 (MQ=255)                                                                                    tttCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGt                                                                   <  1:179251/140‑1 (MQ=255)                                                                                    tttCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGt                                                                   >  2:179251/1‑140 (MQ=255)                                                                                       ttcccAGCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTcagccgca                                                      >  2:98611/3‑150 (MQ=255)                                                                                          ccATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCCTCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAgcagcagca                                                   >  1:207128/1‑150 (MQ=255)                                                                                                   aTGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCt                                          <  1:202327/150‑1 (MQ=255)                                                                                                             gcgACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTgc                                <  2:220976/150‑1 (MQ=255)                                                                                                               gACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTgcac                              >  1:403765/1‑150 (MQ=255)                                                                                                                          gCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGgcgc                   <  2:328020/150‑1 (MQ=255)                                                                                                                                  gCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTGCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTc           <  2:207128/150‑1 (MQ=255)                                                                                                                                         accaGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTc           >  1:75304/1‑143 (MQ=255)                                                                                                                                         accaGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTc           <  2:75304/143‑1 (MQ=255)                                                                                                                                           caGCTCTCCCGGTGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGga  >  2:144869/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                TGCGCCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGGA  >  NC_002947/3816402‑3816689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑