| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,833,384 | C→A | 14.8% | P2P (CCC→CCA) | vgrG‑II → | type VI secretion system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,833,384 | 0 | C | A | 14.8% | 60.5 / 4.7 | 27 | P2P (CCC→CCA) | vgrG‑II | type VI secretion system protein |
| Reads supporting (aligned to +/- strand): ref base C (11/12); new base A (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01 | |||||||||||
GGCCCTGGTGGACCCCGCTGGTCATCTCGGTCATCGCCATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCATCAGCTCCCCGTTCCGACTACAACTGGAGCTGGTCAGTTTCGAAGACGATGTCGACTTCGCCCA > NC_002947/3833238‑3833533 | ggCCCTGGTGGACCCCGCTGGTCATCTCGGTCATCGCCATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGc < 2:405838/150‑1 (MQ=255) tCATCTCGGTCATCGCCATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCAGAAGTCGTCGAATGGCTCAACGCCATTCTGCAGCCGTAAAGGGGAACGCCACACGCACAGCCACTCCGCTCTGCGCtgcggg > 2:191400/1‑145 (MQ=255) cATCTCGGTCATCGCCATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACtt > 2:548906/1‑150 (MQ=255) tctcGGTCATCGCCATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTc < 1:30817/150‑1 (MQ=255) tcGGTCATCGCCATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCa > 2:249990/1‑150 (MQ=255) tCGCCATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTg > 1:490668/1‑150 (MQ=255) cATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTg < 2:490668/150‑1 (MQ=255) gctactacagctacCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGCTTggg > 1:101140/1‑148 (MQ=255) gctaccacagctacCCCCTCCACCTGCTAACCGCCGAAGTCGTCAAATCTCTCAACCCCATTCTGCAGCAGTAACGGGGGATGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAg < 1:229357/150‑1 (MQ=255) tacagctacCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAggtggt < 1:459554/150‑1 (MQ=255) tacagctacCGCCTCGACCTGCTAACCGCCCAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGATTGGCGGTAGCGAGTTTGtggtggg > 1:470004/1‑149 (MQ=255) tGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGaa < 1:249990/150‑1 (MQ=255) cgccgAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCATCa > 1:34724/1‑150 (MQ=255) gtcgAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAggtggt < 2:30531/114‑1 (MQ=255) gtcgAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAggtggt > 1:30531/1‑114 (MQ=255) gtcgAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCAAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCa > 2:203575/1‑137 (MQ=255) gtcgAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCAAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCa > 2:203549/1‑137 (MQ=255) gtcgAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCAAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCa < 1:203549/137‑1 (MQ=255) gtcgAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCAAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCa < 1:203575/137‑1 (MQ=255) aaTCGCGCAACGCAATTCTGAAGAAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGGGGTGGTGTCGTTCGAACTGGACGAAGCCATCAGCTCCCCGTTCCGa < 2:101140/150‑1 (MQ=255) aaCGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATcc > 1:457867/1‑49 (MQ=255) aaCGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATcc < 2:457867/49‑1 (MQ=255) aaCGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGa < 1:407031/90‑1 (MQ=255) aaCGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGa > 2:407031/1‑90 (MQ=255) gTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTg < 2:572465/98‑1 (MQ=255) gTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTg > 1:572465/1‑98 (MQ=255) tAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCATCAGCTCCCCGTTCCGACTACAACTGGAGCTGGTCAGTTTCGa < 1:528452/150‑1 (MQ=255) cAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCCGTTGGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCATCAGCTCCCCGTTCCGACTACAACTGGAGCTGGTCAGTTTCGAAGACGATGTCGACTTCGCCCa > 1:204763/1‑150 (MQ=255) | GGCCCTGGTGGACCCCGCTGGTCATCTCGGTCATCGCCATGGTCTGCGCCTACAGCTACTACAGCTACCGCCTCGACCTGCTAACCGCCGAAGTCGTCGAATCGCTCAACGCCATTCTGCAGCAGTAACGGGGGACGCCACATGCCCAGCCAATCCGATCTGCGCTTCACTTTCCAGCCGCTGGTTGGCCGTAGCGAGTTTGAGGTGGTGTCGTTCGAACTGGACGAAGCCATCAGCTCCCCGTTCCGACTACAACTGGAGCTGGTCAGTTTCGAAGACGATGTCGACTTCGCCCA > NC_002947/3833238‑3833533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |