| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,851,001 | A→C | 12.1% | N147H (AAT→CAT) | PP_3398 → | curli fiber surface‑exposed nucleator CsgB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,851,001 | 0 | A | C | 12.1% | 82.0 / 5.1 | 33 | N147H (AAT→CAT) | PP_3398 | curli fiber surface‑exposed nucleator CsgB |
| Reads supporting (aligned to +/- strand): ref base A (16/13); new base C (2/2); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
AACAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCAGGA > NC_002947/3850861‑3851144 | aaCAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCCATTGCCAGc > 2:460152/1‑150 (MQ=255)aaCAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGc > 1:333796/1‑150 (MQ=255)aaCAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGc > 2:175727/1‑150 (MQ=255) aGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCCATTGCCAGCCTg < 1:460152/150‑1 (MQ=255) tGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGAt > 2:431073/1‑150 (MQ=255) tGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGAt > 2:431048/1‑150 (MQ=255) aCAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCAccc < 2:229942/150‑1 (MQ=255) ccGATTCGCCAGAGCGCCAGCAGCACCACCGCCGCCATCGAGCAGATCGGCAACGACAACCGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGa < 2:586031/149‑1 (MQ=255) aTTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCGGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCATGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAg > 1:586031/1‑150 (MQ=255) ttCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGa > 1:34238/1‑150 (MQ=255) tCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGAc < 1:175727/150‑1 (MQ=255) ccAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGATCAGGTTCCAATAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTg > 1:38475/1‑150 (MQ=255) acaacGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAg > 1:188166/1‑101 (MQ=255) acaacGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAg < 2:188166/101‑1 (MQ=255) ccATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAgctcgct > 1:271732/1‑150 (MQ=255) tCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCg > 2:527142/1‑150 (MQ=255) cGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCTCCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGt < 2:544830/150‑1 (MQ=255) aacaGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCCATTGCCAGcct < 1:577714/72‑3 (MQ=255) aacaGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCCATTGCCAGcct > 2:577714/1‑70 (MQ=255) aacaGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCt < 1:431073/150‑1 (MQ=255) aacaGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCt < 1:431048/150‑1 (MQ=255) gcgcCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGcc > 2:510745/1‑150 (MQ=255) cATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAg < 1:527142/150‑1 (MQ=255) cGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATgg < 1:329214/150‑1 (MQ=255) cTCAGGGCTCAACAGCGCCGTGACCCCGGCTGCCAATCGACAGCATGTTCAGATCACCCAATCCCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTGAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGgctg < 2:271732/150‑1 (MQ=255) tCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGgctgc > 1:343335/1‑150 (MQ=255) tCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGc > 1:70003/1‑150 (MQ=255) cTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGAt > 1:243812/1‑53 (MQ=255) cTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGAt < 2:243812/53‑1 (MQ=255) cccAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGAcc > 1:406401/1‑150 (MQ=255) aGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCGCGGCCCAGACCCAg < 2:38475/150‑1 (MQ=255) gCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCAGGa > 2:203247/1‑150 (MQ=255) gCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCGCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCAGGa < 2:34238/150‑1 (MQ=255) | AACAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCAGGA > NC_002947/3850861‑3851144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |