| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,080,963 | A→T | 15.4% | intergenic (‑143/+20) | amaC ← / ← dpkA | D‑lysine aminotransferase/delta 1‑piperideine‑2‑carboxylate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,080,963 | 0 | A | T | 15.4% | 60.4 / 5.5 | 26 | intergenic (‑143/+20) | amaC/dpkA | D‑lysine aminotransferase/delta 1‑piperideine‑2‑carboxylate reductase |
| Reads supporting (aligned to +/- strand): ref base A (13/9); new base T (2/2); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
CACAGCGTGCAGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTCAGCCCCAC > NC_002947/4080818‑4081099 | cacaGCGTGCAGCTCCTTGGATCAGGACGGTACAGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAAcc < 1:70080/150‑1 (MQ=255) gCAGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAgg < 2:154771/150‑1 (MQ=255) ggACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAag > 2:223127/1‑150 (MQ=255) ggAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCttt < 2:65493/150‑1 (MQ=255) gAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTCTTCGCGGGTTTACCCGCGaaaaa > 1:339693/1‑122 (MQ=255) gAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTCTTCGCGGGTTTACCCGCGaaaaa < 2:339693/125‑4 (MQ=255) gTCAGTGTTGAGCATACCCGGAGTGCGGGTTCACGAACAACCACTATTGTGAAAGGTTGTGTATGCAATATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGc < 1:54130/150‑1 (MQ=255) aGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCtt < 1:223127/150‑1 (MQ=255) gggTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTcc < 2:542656/135‑1 (MQ=255) gggTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTcc > 1:542656/1‑135 (MQ=255) gAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCcctc > 1:196623/1‑150 (MQ=255) aCATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCcctcct > 1:501153/1‑150 (MQ=255) tATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGcc > 1:154446/1‑150 (MQ=255) tATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTAGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGAAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGcc > 2:400188/1‑150 (MQ=255) tgCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGTCAAATCAGCCAAGCAGct > 2:186582/1‑92 (MQ=255) tgCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGTCAAATCAGCCAAGCAGct < 1:186582/92‑1 (MQ=255) ggTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCt > 2:472323/1‑150 (MQ=255) ggTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCt > 2:185040/1‑150 (MQ=255) tGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGTCACCTCGCGCTCACGGTAgc > 1:243115/1‑150 (MQ=255) tGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAgc > 1:546925/1‑150 (MQ=255) aTGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGccc > 1:283276/1‑150 (MQ=255) cGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCAt < 1:472323/150‑1 (MQ=255) gAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGt < 1:3455/150‑1 (MQ=255) ctTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTCAGCCCCAc > 1:334912/1‑150 (MQ=255) cccttCGCGGGTTTACCCGCGAAGGGGTCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATgc < 1:6593/133‑1 (MQ=255) cccttCGCGGGTTTACCCGCGAAGGGGTCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATgc > 2:6593/4‑136 (MQ=255) | CACAGCGTGCAGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGATGAGGGCTGGCTTTTTCGCGGGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTCAGCCCCAC > NC_002947/4080818‑4081099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |