Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,221,231	G→T	28.8%	intergenic (+46/+216)	PP_3699 → / ← PP_3700	hypothetical protein/chromosome partitioning ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,221,231	0	G	T	28.8%	23.5 / 6.1	17	intergenic (+46/+216)	PP_3699/PP_3700	hypothetical protein/chromosome partitioning ATPase
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base T (2/3);  total (7/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGTGCAACGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCATCTCTAGCTCCGAGCATGCCCT  >  NC_002947/4221090‑4221366                                                                                                                                              |                                                                                                                                        tGCGTGCAACGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCTGACAGATg                                                                                                                                 <  2:281062/150‑1 (MQ=255)    gTGCAACGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCTGTCCGATGccc                                                                                                                              >  2:497985/1‑150 (MQ=255)     tGCAACGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCTGTCCGATGcccc                                                                                                                             <  1:497985/150‑1 (MQ=255)                   gACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAAcctcagcctcag                                                                                                               <  2:246267/150‑1 (MQ=255)                                          tGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTAt                                                                                        >  1:453374/1‑150 (MQ=255)                                                    aTCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAAcctcagc                                                                                                                    <  2:478226/112‑1 (MQ=255)                                                    aTCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAAcctcagc                                                                                                                    >  1:478226/1‑112 (MQ=255)                                                          gCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGaa                                                                        <  1:180854/150‑1 (MQ=255)                                                           cTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAg                                                                       <  2:453374/150‑1 (MQ=255)                                                                                     gACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCa                                                            >  1:463829/1‑135 (MQ=255)                                                                                     gACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCa                                                            <  2:463829/135‑1 (MQ=255)                                                                                                     gCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTa                             <  1:295015/150‑1 (MQ=255)                                                                                                                                gcTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCATCTCTAGCTCCGAGCATGCCCt  >  2:315003/1‑150 (MQ=255)                                                                                                                                  tCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGc                                                                                     >  1:302680/1‑65 (MQ=255)                                                                                                                                  tCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGc                                                                                     <  2:302680/65‑1 (MQ=255)                                                                                                                                    ttggggCATCTGTCCGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCa                        >  2:174790/3‑124 (MQ=255)                                                                                                                                    ttggggCATCTGTCCGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCa                        <  1:174790/122‑1 (MQ=255)                                                                                                                                              |                                                                                                                                        TGCGTGCAACGTCGAGTTGACGCTGTTCACGCTGAAGATCCTGAAGCTTTGATCAGGGCTGATATGCTTGAGGAGTTTGAACAAGACTGCAAGTGAAGTGGCAAATGCTGACCGTCGTGCATAGCGCGCTCAGGGGGCATCGGACAGATGCCCCAACCTCAGCCTCAGCCTCAGCCTCAAAACCTAGCTATGGCCGAGCGCGCCGAAGCCACAATCTCATGCAACCGCTGCCGTACGAGTCGGCACAGTATTGCATCTCTAGCTCCGAGCATGCCCT  >  NC_002947/4221090‑4221366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑