Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,338,437	T→A	16.1%	intergenic (+13/+129)	PP_3812 → / ← PP_5632	lipase/allo‑isoleucine metabolism protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,338,437	0	T	A	16.1%	75.5 / 8.3	31	intergenic (+13/+129)	PP_3812/PP_5632	lipase/allo‑isoleucine metabolism protein
Reads supporting (aligned to +/- strand):  ref base T (11/15);  new base A (3/2);  total (14/17)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

CGTGACAGTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACC  >  NC_002947/4338292‑4338579                                                                                                                                                  |                                                                                                                                               cgTGACAGTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGAc                                                                                                                                            <  1:469842/150‑1 (MQ=255)        gaaaCCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCCACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACCAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTg                                                                                                                                     <  1:220938/148‑1 (MQ=255)         tAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTgg                                                                                                                                    >  1:388057/1‑150 (MQ=255)            ccTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCg                                                                                                                                 >  1:331760/1‑150 (MQ=255)             cTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAAAAGACCCGCCCAGGCgg                                                                                                                                >  1:92396/1‑150 (MQ=255)                   gcgcTACAACGGACTGATTCGTGACGATGGGCTGCTCAACCCGGTGAGCCAAGTGACGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGGCACGAACGTAAAGTGCATCTGCAGTGTGGCGATGAGCAATAGACCCGCCTGGGCGGATCttt                                                                                                                          <  2:99841/150‑1 (MQ=255)                    cgcTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCtatt                                                                                                                         <  2:92396/150‑1 (MQ=255)                       tACAACGGAATGATTCATGACGATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGCAACTTATACCCGCCTGGGCGGGTCTTTTGCt                                                                                                                      >  1:308371/1‑150 (MQ=255)                                   aTTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGa                                                                                                          >  2:347204/1‑150 (MQ=255)                                   aTTCATGACTAGGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGGGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGa                                                                                                          <  1:12696/150‑1 (MQ=255)                                              tGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCa                                                                                               <  1:347204/150‑1 (MQ=255)                                                ggCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAgg                                                                                             <  2:324452/150‑1 (MQ=255)                                                      tCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCaa                                                                                       <  2:170729/150‑1 (MQ=255)                                                       cAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAAt                                                                                      <  2:331760/150‑1 (MQ=255)                                                           ccGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAAt                                                                                  >  1:557832/1‑150 (MQ=255)                                                                  gCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAg                                                                           >  2:129061/1‑150 (MQ=255)                                                                       gTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGAccc                                                                                                                                          >  1:424912/1‑82 (MQ=255)                                                                       gTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGAccc                                                                                                                                          <  2:424912/82‑1 (MQ=255)                                                                          ccGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCgg                                                                   <  2:557832/150‑1 (MQ=255)                                                                           cGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCTATTg                                                                                                                        >  1:223011/1‑96 (MQ=38)                                                                           cGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCTATTg                                                                                                                        <  2:223011/96‑1 (MQ=38)                                                                             gCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTTGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAg                                                                >  1:172917/1‑150 (MQ=255)                                                                               aGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGt                                                              <  2:592550/150‑1 (MQ=255)                                                                                    aGGCGGCGCTGCGGCAGGCGGGCAACGAGCTTAGAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCGGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGc                                                         <  1:107218/150‑1 (MQ=255)                                                                                              gCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCgg                                               <  2:315836/150‑1 (MQ=255)                                                                                                          cAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGa                                   <  2:173358/150‑1 (MQ=255)                                                                                                           aaCGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGaa                                  >  1:432897/1‑150 (MQ=255)                                                                                                                aCTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGc                             >  1:387289/1‑150 (MQ=255)                                                                                                                                  gTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGc                                                                     >  2:352773/1‑92 (MQ=255)                                                                                                                                  gTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGc                                                                     <  1:352773/92‑1 (MQ=255)                                                                                                                                           gaaGCAAAAGACCCGCCCAGGCGGGTCTATTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAAcc  >  1:201734/3‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                               CGTGACAGTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACC  >  NC_002947/4338292‑4338579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑