Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,338,447 T→C 16.3% intergenic (+23/+119) PP_3812 → / ← PP_5632 lipase/allo‑isoleucine metabolism protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,338,4470TC16.3% 64.9 / 5.3 31intergenic (+23/+119)PP_3812/PP_5632lipase/allo‑isoleucine metabolism protein
Reads supporting (aligned to +/- strand):  ref base T (11/14);  new base C (3/2);  total (14/17)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAA  >  NC_002947/4338299‑4338588
                                                                                                                                                    |                                                                                                                                             
gaaaCCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCCACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACCAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTg                                                                                                                                              <  1:220938/148‑1 (MQ=255)
 tAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTgg                                                                                                                                             >  1:388057/1‑150 (MQ=255)
    ccTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCg                                                                                                                                          >  1:331760/1‑150 (MQ=255)
     cTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAAAAGACCCGCCCAGGCgg                                                                                                                                         >  1:92396/1‑150 (MQ=255)
           gcgcTACAACGGACTGATTCGTGACGATGGGCTGCTCAACCCGGTGAGCCAAGTGACGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGGCACGAACGTAAAGTGCATCTGCAGTGTGGCGATGAGCAATAGACCCGCCTGGGCGGATCttt                                                                                                                                   <  2:99841/150‑1 (MQ=255)
            cgcTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCtatt                                                                                                                                  <  2:92396/150‑1 (MQ=255)
               tACAACGGAATGATTCATGACGATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGCAACTTATACCCGCCTGGGCGGGTCTTTTGCt                                                                                                                               >  1:308371/1‑150 (MQ=255)
                           aTTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGa                                                                                                                   >  2:347204/1‑150 (MQ=255)
                           aTTCATGACTAGGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGGGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGa                                                                                                                   <  1:12696/150‑1 (MQ=255)
                                      tGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCa                                                                                                        <  1:347204/150‑1 (MQ=255)
                                        ggCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAgg                                                                                                      <  2:324452/150‑1 (MQ=255)
                                              tCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCaa                                                                                                <  2:170729/150‑1 (MQ=255)
                                               cAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAAt                                                                                               <  2:331760/150‑1 (MQ=255)
                                                   ccGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAAt                                                                                           >  1:557832/1‑150 (MQ=255)
                                                          gCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAg                                                                                    >  2:129061/1‑150 (MQ=255)
                                                                  ccGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCgg                                                                            <  2:557832/150‑1 (MQ=255)
                                                                   cGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCTATTg                                                                                                                                 >  1:223011/1‑96 (MQ=38)
                                                                   cGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCTATTg                                                                                                                                 <  2:223011/96‑1 (MQ=38)
                                                                     gCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTTGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAg                                                                         >  1:172917/1‑150 (MQ=255)
                                                                       aGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGt                                                                       <  2:592550/150‑1 (MQ=255)
                                                                            aGGCGGCGCTGCGGCAGGCGGGCAACGAGCTTAGAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCGGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGc                                                                  <  1:107218/150‑1 (MQ=255)
                                                                                      gCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCgg                                                        <  2:315836/150‑1 (MQ=255)
                                                                                                  cAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGa                                            <  2:173358/150‑1 (MQ=255)
                                                                                                   aaCGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGaa                                           >  1:432897/1‑150 (MQ=255)
                                                                                                        aCTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGc                                      >  1:387289/1‑150 (MQ=255)
                                                                                                                          gTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGc                                                                              >  2:352773/1‑92 (MQ=255)
                                                                                                                          gTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGc                                                                              <  1:352773/92‑1 (MQ=255)
                                                                                                                                   gaaGCAAAAGACCCGCCCAGGCGGGTCTATTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAAcc           >  1:201734/3‑150 (MQ=255)
                                                                                                                                            gACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACaa  <  1:129061/150‑1 (MQ=255)
                                                                                                                                            gACCCGCCTGGGCGGGTCTCTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGaaa                                          >  1:459767/1‑110 (MQ=255)
                                                                                                                                            gACCCGCCTGGGCGGGTCTCTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGaaa                                          <  2:459767/110‑1 (MQ=39)
                                                                                                                                                    |                                                                                                                                             
GTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAA  >  NC_002947/4338299‑4338588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑