| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,357,791 | G→A | 20.1% | intergenic (+93/‑161) | PP_3831 → / → PP_3832 | hypothetical protein/CsrA‑like carbon storage regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,357,791 | 0 | G | A | 20.1% | 40.7 / 7.5 | 25 | intergenic (+93/‑161) | PP_3831/PP_3832 | hypothetical protein/CsrA‑like carbon storage regulator |
| Reads supporting (aligned to +/- strand): ref base G (10/10); new base A (2/3); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
CTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATA > NC_002947/4357653‑4357932 | cTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCTAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTgtcg < 1:227981/150‑4 (MQ=255) tttttATTCAGGCACTTGAGGAATAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCt < 2:229299/148‑1 (MQ=255) gCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGa < 1:567798/150‑1 (MQ=255) aCTTGAGGAACAGGAGGACCATTGAGCTGGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGaca > 1:172789/1‑150 (MQ=255) tGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGCAATACGTTGGCACCTGCAACTGAAGACACGc < 1:476171/150‑1 (MQ=255) gAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGCAGACACGCa > 2:209365/1‑150 (MQ=255) aCAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGa > 1:413861/1‑150 (MQ=255) aTTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTtgtctgtc > 1:337350/1‑150 (MQ=255) gAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCggg < 2:223710/150‑1 (MQ=255) cTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTg > 2:69146/1‑95 (MQ=255) cTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTg < 1:69146/95‑1 (MQ=255) agaaATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTa > 2:284608/1‑150 (MQ=255) tCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATTCGCCGAAAGCGTCTTTAATGAgaa < 2:413861/150‑1 (MQ=255) aTTAGCCGCCAACCCCCCTATTCTTGCCAGCGCGCACCTGGCGCTACAAACCGTGGACGGTTTGCTTTTGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTCTAAGGAgaaggaa > 1:502588/1‑150 (MQ=255) gccgccAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAgg < 2:502588/150‑1 (MQ=255) cAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAAAAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACaa < 2:337350/150‑1 (MQ=255) aaCCCCCCTATTCTTGCCAGGAAGCCCCTTGGCCGATAAACCGTTGACGGTTTACTCTGGGACCTGGAAGTGAAGACACGCAACAGTATTTGTCTGTCGGGGAATTAGTATCCGCCCAAAGAGTCTTTAATTAGAACGAAGAGGGACAAg < 2:187444/150‑1 (MQ=255) cccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGTTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAGGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTccc > 2:328665/1‑150 (MQ=255) tATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGaa < 1:529468/66‑1 (MQ=255) tATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGaa > 2:529468/1‑66 (MQ=255) gCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCgg > 2:47743/1‑150 (MQ=255) aaagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATga > 2:117998/5‑101 (MQ=255) aaagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATga < 1:117998/97‑1 (MQ=255) cGACAAACCGTTGACCGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACt < 1:367615/150‑1 (MQ=255) aaaCCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATACGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTtata > 2:214007/1‑150 (MQ=255) | CTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATA > NC_002947/4357653‑4357932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |