| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,410,822 | G→C | 21.0% | H121Q (CAC→CAG) | PP_3894 ← | replication protein O |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,410,822 | 0 | G | C | 21.0% | 36.4 / 5.5 | 19 | H121Q (CAC→CAG) | PP_3894 | replication protein O |
| Reads supporting (aligned to +/- strand): ref base G (9/6); new base C (2/2); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GGTTGCCTTGTCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCG > NC_002947/4410678‑4410965 | ggTTGCCTTGTCGTTGCCGTGACCTCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAgtgtgt < 2:191406/150‑1 (MQ=255) cTTGTCGTTGCCATGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGAttt > 1:425627/1‑150 (MQ=255) gCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCt < 2:366921/150‑1 (MQ=255) gTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTCTTTATGTTTCTCATTCAGAAGGACGGAAGTGTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTTCTGTCGATTCTCCACTCa < 1:227398/150‑1 (MQ=255) tCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGaaa > 1:462476/1‑150 (MQ=255) tCGACAACCAATGCGTTAGCATTGGAATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATGTCAACACGGTTGGCTTGCTGTCGATTCTCCACGCAGGTACCGGTAGGaaa > 1:153891/1‑150 (MQ=255) ccAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTg > 2:41512/1‑150 (MQ=255) gCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAgtgt < 2:149467/81‑1 (MQ=255) gCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAgtgt > 1:149467/1‑81 (MQ=255) tGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTgccgc < 1:41512/150‑1 (MQ=255) tttgtatttCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcg > 1:425379/1‑150 (MQ=255) ttgtatttCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcgc < 2:462476/150‑1 (MQ=255) tatgtAATTTTCAACACAGTGGAAGGGGTGTTTTCTAAACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTACACTCAGATAACGGTAGGAAAGTGATTGGCTCGCGACTGCCGCCATAGCGGAAAAGCACGCGCTGACgg < 2:33903/148‑1 (MQ=255) tgtAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGAt > 1:5262/1‑150 (MQ=255) tgtAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGAt > 1:591080/1‑150 (MQ=255) acacAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCGGCACGGGTGGCTTGCTTAAAAATCTAAACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGa > 2:235080/1‑150 (MQ=255) tGGCAGGGGTTTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGccc < 2:434074/150‑1 (MQ=255) aatCTACACACTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGAtt < 2:208586/75‑1 (MQ=255) aatCTACACACTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGAtt < 2:208625/75‑1 (MQ=255) aatCTACACACTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGAtt > 1:208625/3‑77 (MQ=255) aatCTACACACTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGAtt > 1:208586/3‑77 (MQ=255) tACACAGTGTGTAGATTTCAGTACTGGTGGCTTCCTTTCAATACTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGgcgcg > 2:254611/1‑150 (MQ=255) | GGTTGCCTTGTCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCG > NC_002947/4410678‑4410965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |