Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,470,281 G→A 8.5% T318I (ACC→ATC)  PP_3961 ← transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,470,2810GA 8.5% 128.7 / 3.5 47T318I (ACC→ATC) PP_3961transporter
Reads supporting (aligned to +/- strand):  ref base G (19/24);  new base A (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

GCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAGGACAC  >  NC_002947/4470137‑4470428
                                                                                                                                                |                                                                                                                                                   
gCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCggg                                                                                                                                                <  2:493167/150‑1 (MQ=255)
gCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCggg                                                                                                                                                <  1:245990/150‑1 (MQ=255)
               cccaccTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGTGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGccc                                                                                                                                 <  1:48000/149‑1 (MQ=255)
               accaccTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGccc                                                                                                                                 <  1:209290/150‑1 (MQ=255)
                  accTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCa                                                                                                                              <  2:211695/150‑1 (MQ=255)
                      gCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCa                                                                                                                          >  2:516499/1‑150 (MQ=255)
                         cAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCa                                                                                                                       >  1:310383/1‑150 (MQ=255)
                         cAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCa                                                                                                                       >  1:414048/1‑150 (MQ=255)
                              gctgcGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGc                                                                                                                                              <  1:156098/122‑1 (MQ=255)
                              gctgcGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGc                                                                                                                                              >  2:156098/1‑122 (MQ=255)
                                   ggCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGc                                                                                                             <  1:572073/150‑1 (MQ=255)
                                     cACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCa                                                                                                           <  1:516499/150‑1 (MQ=255)
                                         gccgGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGccc                                                                                                       >  1:264932/1‑150 (MQ=255)
                                                  tCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc                                                                                                                            <  2:98619/120‑1 (MQ=255)
                                                  tCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc                                                                                                                            >  1:98619/1‑120 (MQ=255)
                                                   ccAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGttt                                                                                             <  2:310383/150‑1 (MQ=255)
                                                    cAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGCTGATGTTTc                                                                                            >  2:17041/1‑150 (MQ=255)
                                                                  gCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCagcagc                                                                              >  2:462147/1‑150 (MQ=255)
                                                                       cAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCaa                                                                         <  2:414048/150‑1 (MQ=255)
                                                                       cAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCaa                                                                         >  1:55439/1‑150 (MQ=255)
                                                                        aaCCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGc                                                                                                                                              <  1:581358/80‑1 (MQ=38)
                                                                        aaCCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGc                                                                                                                                              >  2:581358/1‑80 (MQ=38)
                                                                        aaCCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg                                                                        <  2:55439/150‑1 (MQ=255)
                                                                         aCCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAgg                                                                       >  1:513942/1‑150 (MQ=255)
                                                                          ccAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCgg                                                                                                                                                 >  2:535263/1‑75 (MQ=255)
                                                                          ccAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCgg                                                                                                                                                 <  1:535263/75‑1 (MQ=255)
                                                                                 aTCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCgg                                                                                                                                                 >  1:328749/1‑68 (MQ=255)
                                                                                 aTCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCgg                                                                                                                                                 <  2:328749/68‑1 (MQ=255)
                                                                                        cGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTCTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCg                                                        >  1:188584/1‑150 (MQ=255)
                                                                                           tCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCGGCGCTCACTGCGATGGCCAGCCCGATGATGTTTCACACCATCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCa                                                     >  1:564864/1‑150 (MQ=255)
                                                                                                 tgcttTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAacccac                                               <  2:122607/150‑1 (MQ=255)
                                                                                                          gCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg                                                                        <  1:58165/116‑1 (MQ=255)
                                                                                                          gCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg                                                                        >  2:58165/1‑116 (MQ=255)
                                                                                                              gATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTagcag                                  >  2:345499/1‑150 (MQ=255)
                                                                                                                   gcTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTtgt                             <  2:513942/150‑1 (MQ=255)
                                                                                                                   gcTCTACCACCTACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGAAGTAGCAGCTtgt                             >  2:609513/1‑150 (MQ=255)
                                                                                                                          cAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGc                                                                                                                                   >  1:229444/1‑41 (MQ=255)
                                                                                                                          cAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGc                                                                                                                                   <  2:229444/41‑1 (MQ=255)
                                                                                                                          cAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCgg                      <  1:17041/150‑1 (MQ=255)
                                                                                                                           aGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCggg                     <  2:34213/150‑1 (MQ=255)
                                                                                                                             cGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGt                   <  2:188584/150‑1 (MQ=255)
                                                                                                                                  tCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCgt              <  2:421209/150‑2 (MQ=255)
                                                                                                                                      cACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGttt          >  2:348687/1‑150 (MQ=255)
                                                                                                                                         gATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAg       <  1:462147/150‑1 (MQ=255)
                                                                                                                                              tGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTagc                                    <  2:504113/116‑1 (MQ=255)
                                                                                                                                              tGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTagc                                    >  1:504113/1‑116 (MQ=255)
                                                                                                                                              tGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAGGacac  <  2:564864/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAGGACAC  >  NC_002947/4470137‑4470428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑