| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,532,626 | T→C | 12.9% | M105T (ATG→ACG) ‡ | PP_4020 → | oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,532,626 | 0 | T | C | 12.9% | 73.8 / 4.0 | 31 | M105T (ATG→ACG) ‡ | PP_4020 | oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (14/13); new base C (2/2); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01 | |||||||||||
CGTAACGCTGCTGGAAGCCAGCCGGGTTGCCTGGGCGGCTTCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGAAGAGGCCCTGCATGATGC > NC_002947/4532483‑4532758 | cgTAACGCTGCTGGAAGCCAGCCGGGTTGCCTGGGCGGCTTCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTg < 2:200019/150‑1 (MQ=255) tgctgGAAGCCAGCCGGGTTGCCTGGGCGGCTTCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGccgccg > 2:173949/1‑150 (MQ=255) ccTGGGCGGCTTCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGCCCAGCACATGCTggcc < 2:94423/124‑3 (MQ=255) ccTGGGCGGCTTCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGCCCAGCACATGCTggcc > 1:94423/1‑122 (MQ=255) ggcggcTTCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACg > 2:385192/1‑150 (MQ=255) cTTCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACg < 1:173949/150‑1 (MQ=255) ttCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACgg > 2:451522/1‑150 (MQ=255) ttCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACgg < 1:265747/150‑1 (MQ=255) aaCGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATc > 2:243626/1‑150 (MQ=255) aaCGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGCCCAGCACATGCTggccca > 1:446648/1‑102 (MQ=255) aaCGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGCCCAGCACATGCTGGccca < 2:446648/106‑2 (MQ=255) cGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGa < 2:20111/150‑1 (MQ=255) gTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGc > 2:397813/1‑150 (MQ=255) aTGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTg > 2:348202/1‑150 (MQ=255) gccgccGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGcc > 1:88720/1‑150 (MQ=255) gCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGaa > 1:456622/1‑150 (MQ=255) gCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCtgt < 1:606997/118‑1 (MQ=255) gCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCtgt > 2:606997/1‑118 (MQ=255) ccTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATc > 1:544196/1‑94 (MQ=255) ccTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATc < 2:544196/94‑1 (MQ=255) cTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTc < 1:348202/150‑1 (MQ=255) ggCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGaa < 1:451522/150‑1 (MQ=255) cATCGAACGCACCGAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGAaga < 1:29381/150‑1 (MQ=255) tCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGAAGAgg < 1:385192/150‑1 (MQ=255) tCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCAACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTCTTGCCGCGCCGCGCGAAAATGCTCGAagagc > 2:276217/1‑149 (MQ=255) gCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGAAGAGGCCCTGCATGATGc > 1:60145/1‑140 (MQ=255) gCCAGCATGGGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGAAGAGGCCCTGCATGATGc < 2:60145/140‑1 (MQ=255) cAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGAag < 2:521908/122‑1 (MQ=255) cAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGAag > 1:521908/1‑122 (MQ=255) gCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTAcc > 2:471489/1‑66 (MQ=255) gCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTAcc < 1:471489/66‑1 (MQ=255) | CGTAACGCTGCTGGAAGCCAGCCGGGTTGCCTGGGCGGCTTCCGGACGCAACGGTGGCCAGGCGCTGTTGGGCTGGTCGTGCGACATGCCGCCGCTGGAGCAGGCCCTTGGCATCGAACGCACCCAACGGCTATGGGCCAGCATGTGCTGGGCCGCCGAAGAGATGCGCGAACTGCCCCAACGCCACGGCTTCGACATCGACTACCGGCAGGGCAGCCTGTGGACTGCGGTATTGCCGCGCCGGGTGAAAATGCTCGAAGAGGCCCTGCATGATGC > NC_002947/4532483‑4532758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |