Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,553,312 A→T 9.5% L553Q (CTG→CAG)  PP_4041 ← glycoside hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,553,3120AT 9.5% 122.5 / 4.4 42L553Q (CTG→CAG) PP_4041glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base A (19/19);  new base T (2/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TACTCATTCGCTACCCACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGCT  >  NC_002947/4553163‑4553460
                                                                                                                                                     |                                                                                                                                                    
tACGCATCCGCTACCCACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCca                                                                                                                                                      <  1:93303/150‑1 (MQ=255)
   tCATTCGCTACCCACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCcagca                                                                                                                                                   >  2:173950/1‑150 (MQ=255)
   tCATTCGCTACCCACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCcagca                                                                                                                                                   >  1:273203/1‑150 (MQ=255)
      ttCGCTACCCACAACGCGCCCGGTCGCNCGCCGGGCACTGNGCGCGGNGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGc                                                                                                                                                >  1:462379/1‑150 (MQ=255)
      ttCGCTACCCACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGc                                                                                                                                                <  1:173950/150‑1 (MQ=255)
      ttCGCTACCCACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCT‑GGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGcc                                                                                                                                               >  1:75523/1‑150 (MQ=255)
         gCTACCCACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCT‑GGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAg                                                                                                                                            <  2:75523/150‑1 (MQ=255)
              ccACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCg                                                                                                                                        >  2:268577/1‑150 (MQ=255)
              ccACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCg                                                                                                                                        >  2:523662/1‑150 (MQ=255)
                  aaCGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGc                                                                                                                                    >  1:55822/1‑150 (MQ=255)
                              cgcgggCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGCTGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCGGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCa                                                                                                                        <  1:242576/150‑1 (MQ=255)
                                 gcgcCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGgt                                                                                                                     <  2:462379/150‑1 (MQ=255)
                                      gggCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTgg                                                                                                                <  2:107907/150‑1 (MQ=255)
                                             gtgCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCg                                                                                                                            >  2:607480/1‑131 (MQ=255)
                                             gtgCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCg                                                                                                                            <  1:607480/131‑1 (MQ=255)
                                                 gcgGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTg                                                                                                                 <  2:522840/138‑1 (MQ=255)
                                                 gcgGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTg                                                                                                                 >  1:522840/1‑138 (MQ=255)
                                                 gcgGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTc                                                                                                     <  2:76863/150‑1 (MQ=255)
                                                        aGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTg                                                                                              <  2:605531/150‑1 (MQ=255)
                                                             cAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTg                                                                                         <  1:545798/150‑1 (MQ=255)
                                                               gcgcGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAg                                                                                                                                            >  2:185382/1‑97 (MQ=255)
                                                               gcgcGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAg                                                                                                                                            <  1:185382/97‑1 (MQ=255)
                                                                               gCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGACGTCCTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGATTTGGCCTGGCGCCCCAGTAACACATa                                                                       >  1:230037/1‑150 (MQ=255)
                                                                                  tATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTCCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAAc                                                                    <  2:518517/150‑1 (MQ=255)
                                                                                      tGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTca                                                                <  1:268577/150‑1 (MQ=255)
                                                                                          tGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAAc                                                                    <  1:540058/142‑1 (MQ=255)
                                                                                          tGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAAc                                                                    >  2:540058/1‑142 (MQ=255)
                                                                                                  tGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAgaac                                                    >  1:375396/1‑150 (MQ=255)
                                                                                                    ggggAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAgaacga                                                  >  1:271316/1‑150 (MQ=255)
                                                                                                       gAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAaca                                               <  2:375396/150‑1 (MQ=255)
                                                                                                            tGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCa                                          <  1:103571/150‑1 (MQ=255)
                                                                                                            tGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCa                                          >  2:383453/1‑150 (MQ=255)
                                                                                                              ccGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGACTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAgg                                        >  1:496460/1‑150 (MQ=255)
                                                                                                                    aTGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTg                                  >  1:526339/1‑150 (MQ=255)
                                                                                                                    aTGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTg                                  <  2:526339/150‑1 (MQ=255)
                                                                                                                             ccggccggGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTccc                         <  2:273203/150‑1 (MQ=255)
                                                                                                                               ggccggGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCgg                       >  1:475381/1‑150 (MQ=255)
                                                                                                                                    ggTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCGGCGTCa                  >  2:75603/1‑150 (MQ=255)
                                                                                                                                        gTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCGGCGTCAGGcc              <  1:383453/150‑1 (MQ=255)
                                                                                                                                         tACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCGGCGTCAGGCCg             >  2:553507/1‑150 (MQ=255)
                                                                                                                                             cctCGGCCTGCTGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCGGCGTCAGGCCGTCGc         >  1:552008/3‑150 (MQ=255)
                                                                                                                                              ctCGGCCTGCTGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCa                                          <  1:100414/115‑1 (MQ=255)
                                                                                                                                              ctCGGCCTGCTGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCa                                          >  2:100414/2‑116 (MQ=255)
                                                                                                                                               tCGGCCTGCTGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCGGCGTCAGGCCGTCGCTg       <  2:552008/150‑1 (MQ=255)
                                                                                                                                                   ccAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAAAACACCAGGAAGGTGACCTCTCGCGGCGTCAGGCCGTCGCtcgagt   >  2:272474/1‑145 (MQ=255)
                                                                                                                                                    cagcagGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGct  >  1:556075/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TACTCATTCGCTACCCACAACGCGCCCGGTCGCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTGCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGCT  >  NC_002947/4553163‑4553460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑