| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,169,782 | T→A | 23.1% | L451Q (CTG→CAG) | PP_4547 → | glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,169,782 | 0 | T | A | 23.1% | 47.4 / 13.3 | 26 | L451Q (CTG→CAG) | PP_4547 | glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base T (11/9); new base A (3/3); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01 | |||||||||||
TGGCTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGG > NC_002947/5169634‑5169919 | tgGCTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTg < 2:553901/150‑1 (MQ=255) cGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGTGCAGGTACCAGCGCCAgt < 1:609436/149‑2 (MQ=255) agCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGTGCAGGTACCAGCGCCAgt > 2:609436/2‑148 (MQ=255) cACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCt < 1:250710/150‑1 (MQ=255) aCGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAGTCCTgttcgc < 1:203146/150‑6 (MQ=38) tGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAt > 1:186867/1‑150 (MQ=255) aTTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAg > 1:446439/1‑150 (MQ=255) ccGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGaa > 2:590202/1‑150 (MQ=255) cGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTg > 1:405431/1‑119 (MQ=255) cGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTg < 2:405431/119‑1 (MQ=255) cGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGACCTGAGCCCCTAAATAAAAAGGAACACCCATGAAc > 1:284260/1‑150 (MQ=255) aTTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCAtt < 2:186867/150‑1 (MQ=255) ttCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGTCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCg < 2:301038/150‑1 (MQ=255) ttCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCt > 1:338320/1‑97 (MQ=255) ttCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCt < 2:338320/97‑1 (MQ=255) ttCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCg > 2:14489/1‑150 (MQ=255) ccTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAGTCCTgt < 1:174828/97‑2 (MQ=25) ccTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAGTCCTgt > 2:174828/1‑96 (MQ=25) gcgcgcCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTcc > 2:516913/1‑142 (MQ=255) gcgcgcCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTcc < 1:516913/142‑1 (MQ=255) gAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAGTAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACAACACTGCGTACCCGCAACTc > 2:17218/1‑150 (MQ=255) tcAGGCCTGGTGCAGGTACCAGCGCCAGTCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCaa > 2:297337/2‑150 (MQ=255) aCAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCaa > 1:393027/1‑150 (MQ=255) gACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGgcg < 2:170490/84‑1 (MQ=255) gACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGgcg > 1:170490/1‑84 (MQ=255) ggCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACgg < 2:446439/150‑1 (MQ=255) | TGGCTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGG > NC_002947/5169634‑5169919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |