Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	240,536	G→C	22.2%	G277G (GGG→GGC)	PP_0188 →	uroporphyrin‑III C‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	240,536	0	G	C	22.2%	29.6 / 7.1	18	G277G (GGG→GGC)	PP_0188	uroporphyrin‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (7/7);  new base C (2/2);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

CCCGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGC  >  NC_002947/240387‑240673                                                                                                                                                      |                                                                                                                                          accGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCggg                                                                                                                                           >  1:424422/2‑150 (MQ=255)          gACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTg                                                                                                                                  >  2:385672/1‑150 (MQ=255)                           gcTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGCCCGGCCAGCaa                                                                                                                                     >  1:333870/1‑130 (MQ=255)                           gcTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGCCCGGCCAGCaa                                                                                                                                     <  2:333870/130‑1 (MQ=255)                                gcgcCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGCAATATCCATATACTTCCAGAGCGAACTCCATGCCGATGAAACCGTGCGTCCGTTTGTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGa                                                                                                            <  1:140637/150‑1 (MQ=255)                                                            cagcagGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGggc                                                                                <  1:249232/150‑1 (MQ=255)                                                            cagcagGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGggc                                                                                >  2:249232/1‑150 (MQ=255)                                                                 ggCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGgcgc                                                                           <  2:6945/150‑1 (MQ=255)                                                                                               aGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGTGCGAGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAACAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAgg                                             >  1:417819/1‑150 (MQ=255)                                                                                                         gATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGgacga                                   >  2:512980/1‑150 (MQ=255)                                                                                                            cGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAACGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGc                                <  2:424422/150‑1 (MQ=255)                                                                                                               cTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTg                                                                                                                                  <  1:265698/49‑1 (MQ=255)                                                                                                               cTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTg                                                                                                                                  >  2:265698/1‑49 (MQ=255)                                                                                                                            gAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTActgctg                <  1:385672/150‑1 (MQ=255)                                                                                                                                         cGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCa      >  2:95995/1‑147 (MQ=255)                                                                                                                                         cGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCa      <  1:95995/147‑1 (MQ=255)                                                                                                                                         agTTGCTGCCCGGCCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGc                                                >  1:94503/2‑105 (MQ=255)                                                                                                                                         agTTGCTGCCCGGCCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGc                                                <  2:94503/104‑1 (MQ=255)                                                                                                                                          gTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGc  >  1:93971/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                          CCCGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGC  >  NC_002947/240387‑240673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑