| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 27,584 | A→G | 19.3% | intergenic (‑431/+15) | PP_0022 ← / ← PP_0023 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 27,584 | 0 | A | G | 19.3% | 45.7 / 6.5 | 26 | intergenic (‑431/+15) | PP_0022/PP_0023 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (11/10); new base G (3/2); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
CACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATGCA > NC_002947/27450‑27730 | cACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCt > 1:465305/1‑150 (MQ=255) cGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCGGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCtttttt > 2:327479/1‑149 (MQ=255) ggTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTAACGACGCGGTACGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCGCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTgg < 1:15574/150‑1 (MQ=255) gTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTggg > 1:142543/1‑150 (MQ=255) ttCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTc > 2:44698/1‑150 (MQ=255) ttCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTc < 2:142543/150‑1 (MQ=255) aaTCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTc < 2:317725/150‑1 (MQ=255) aagcGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTc < 1:44698/150‑1 (MQ=255) tCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGCCC‑TTCTGCTTTTCAgg < 1:523258/150‑1 (MQ=255) cgGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTc < 2:429585/150‑1 (MQ=255) tCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGt > 2:609159/1‑150 (MQ=255) gCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCg > 1:290134/1‑150 (MQ=255) gagaCGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGa > 1:376746/1‑150 (MQ=255) gaCGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGt > 2:318868/1‑150 (MQ=255) gCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTgg < 2:465305/150‑1 (MQ=255) aaaaaGCAGCTCTCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCg > 1:511090/1‑113 (MQ=255) aaaaaGCAGCTCTCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCg < 2:511090/113‑1 (MQ=255) aaaaaGCAGCTCTCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCtt < 1:425770/150‑1 (MQ=255) aaaGCAGCTCTCGGGAGCTGCTTTTTTTTTACTCCCCCTTTTTGTGCTTGTGAGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGGGACTCGCTGCCTTTTGTCCGGAGTGCTATCTTGATTGGTGCGCTTTGGGCCTTGCTTGa > 2:276201/1‑150 (MQ=255) aaGCAGCTCTCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTTTTTTTTTGTGTTTTCAGGGCCGGATGATCC‑TTTTGCTTTTCAGGGGGATCTCGCTGCCTTTTGTCCGCACTGCCATCTTCATTAGTGCGCTTTGGCCCTGTCTTTAt > 2:186901/1‑150 (MQ=255) aaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGAt < 1:403944/150‑1 (MQ=255) gcagcTCCCTAGACCTGCTTTTTTCTTACTCCCCCTTTCTGTTCTTGTGGGGTTTCATGTTCGGATGATCC‑TTCTGCGTTTCAGTGGGGAGTCGCTGGCTTCTGTCCGGAGTGACAGCTTCTTTGGTGAGCTTTTGGCCTGGCTCGATGc > 2:411694/1‑150 (MQ=255) tCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGa > 1:503951/1‑142 (MQ=255) tCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGa < 2:503951/142‑1 (MQ=255) tCCCGAGAGCTGCTTATTTCATACTTCTGCTTTTTTGTCTTGGGGGGTTTCAGGTCCGGATGGTCCTTTATGC‑TGTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATg < 1:318868/150‑1 (MQ=255) ccGAGAGCTGCTTTTTTCTTACTTCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATGCa > 2:590760/1‑150 (MQ=255) | CACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCC‑TTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATGCA > NC_002947/27450‑27730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |