| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 270,845 | T→A | 13.8% | L249Q (CTG→CAG) | PP_0218 → | sensory box protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 270,845 | 0 | T | A | 13.8% | 71.4 / 5.6 | 29 | L249Q (CTG→CAG) | PP_0218 | sensory box protein |
| Reads supporting (aligned to +/- strand): ref base T (14/11); new base A (2/2); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01 | |||||||||||
CAAGCATTCGGAGCAGGAGCTTGCCTACCTGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTGGCGCGCCTGGGCGGCGACGAATTCGGCGTGCTGGTCGA > NC_002947/270696‑270992 | cAAGCATTCGGAGCAGGAGCTTGCCTACCTGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCt < 1:434564/150‑1 (MQ=255) ttCGGAGCAGGAGCTTGCCTACCTGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCa > 1:244637/1‑150 (MQ=255) ttGCCTACCTGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGGGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATaa > 1:194373/1‑150 (MQ=255) cTACCTGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTc > 1:447474/1‑136 (MQ=255) cTACCTGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTc < 2:447474/136‑1 (MQ=255) cTACCTGGCCCACCACGACCCGCTG‑CGGCCATTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAacga > 1:172388/1‑150 (MQ=255) tGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCaga > 2:593660/1‑135 (MQ=255) tGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCaga < 1:593660/135‑1 (MQ=255) ccaccaCGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACt < 1:215416/125‑1 (MQ=255) ccaccaCGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACt > 2:215416/1‑125 (MQ=255) ccaccaCGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTggg > 1:229059/1‑150 (MQ=255) ccGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCAGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCAtt > 1:243/1‑150 (MQ=255) ccGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCAGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCAtt > 1:720/1‑150 (MQ=255) tGACTGGC‑CTGCCCAACCGCCTGCTGTTTCCCCACCGGCTCGAGCCGGAATTGGCGGCGGCGCTGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCg < 2:188100/150‑1 (MQ=255) gC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGTCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGt > 2:255762/1‑150 (MQ=255) gC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGATGCGCCTTGTTGCTGCTGGACCTGGCTCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGt > 1:193049/1‑150 (MQ=255) cGCCTGCTGCTTACCGACCGGCTGCAGCATGCATTGGCAGCGGCGCCGGCCAACAAGCGTGGTTGCGCCTTGTTGCCGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCtg < 1:6719/150‑1 (MQ=255) ttACCGACCGGCTAGAGCAGGCATTGGGCGGGCCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGAGCAATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGc < 2:266139/150‑1 (MQ=255) cgaccgGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCt > 2:319826/1‑150 (MQ=255) ggCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTggg < 2:244637/150‑1 (MQ=255) gCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAacgacg < 2:459984/82‑1 (MQ=255) gCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAacgacg > 1:459984/1‑82 (MQ=255) aGGCCAACAAGCGCTGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGGAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTgg < 2:193049/150‑1 (MQ=255) acaaGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTg < 2:9855/143‑1 (MQ=255) acaaGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTg > 1:9855/1‑143 (MQ=255) gcgGTTGCGCCTTGTTGCTGCTGGACCAGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTGGCGCGCCTGggcg < 2:243/150‑1 (MQ=255) gcgGTTGCGCCTTGTTGCTGCTGGACCAGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTGGCGCGCCTGggcg < 2:720/150‑1 (MQ=255) ctgGACCTGGATCACTTACAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGAGCGTGGTCGGCCGTTGCTTCGACTAATTCGCCGTGCTg > 2:313685/1‑150 (MQ=255) ccTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTGGCGCGCCTGGGCGGCGACGAATTCGGCGTGCTGGTCga > 1:448316/1‑150 (MQ=255) ccTGGATCACTTCCAGAGCATAAACGACGGCCTGGGACATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTGGCGCGCCTGGGCGGCGACGAATTCGGCGTGCTGGTCga < 1:313685/150‑1 (MQ=255) | CAAGCATTCGGAGCAGGAGCTTGCCTACCTGGCCCACCACGACCCGCTGACTGGC‑CTGCCCAACCGCCTGCTGTTTACCGACCGGCTGGAGCAGGCATTGGCGGCGGCGCAGGCCAACAAGCGCGGTTGCGCCTTGTTGCTGCTGGACCTGGATCACTTCCAGAGCATAAACGACGGCCTGGGCCATACCATTGGCGACCAGTTGCTGAAGCTGGTGGGCGAGCGTCTGGGGGAGGTGTTGGGTAACGGTGTGACCCTGGCGCGCCTGGGCGGCGACGAATTCGGCGTGCTGGTCGA > NC_002947/270696‑270992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |