| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,550,087 | T→C | 14.3% | L94P (CTG→CCG) | PP_4881 → | iron ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,550,087 | 0 | T | C | 14.3% | 65.1 / 4.2 | 28 | L94P (CTG→CCG) | PP_4881 | iron ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (14/10); new base C (2/2); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01 | |||||||||||
TGGCGAAGCCATTGCCAAGGCCTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTACGTTGGCGCCAACGGCACCTGGATGGGTATCACCGCCCGTACGCGCA > NC_002947/5549938‑5550206 | tGGCGAAGCCATTGCCAAGGCCTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCt < 1:576045/150‑1 (MQ=255) cATTGCCAAGGCCTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCt > 2:377616/1‑150 (MQ=255) gCCAAGGCCTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGc > 1:568195/1‑150 (MQ=255) ccAAGGCCTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAAACAGCTGGCCAGACCGAGCATTGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCCGAACAACCTGGGCg > 1:75006/1‑150 (MQ=255) ccAAGGCCTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGACGCGACCATCAGCTGGCCAAGCGGGTCGTCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCCGAACAACCTGGGCg > 1:381838/1‑150 (MQ=255) cAAGGCCTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGa > 1:494771/1‑150 (MQ=255) ccTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTgg < 2:456368/150‑1 (MQ=255) ccAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTgg > 1:351504/1‑150 (MQ=255) cAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGc > 1:26684/1‑150 (MQ=255) aCCGTCATTCACTTCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCAAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAg < 2:283664/150‑1 (MQ=255) aCCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCAGCAGGCCCAg < 2:494771/150‑1 (MQ=255) cGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCAc > 2:294897/1‑150 (MQ=255) aTCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTg < 1:377616/150‑1 (MQ=255) aTCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTg > 1:262274/1‑150 (MQ=255) cGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAAc > 1:518535/1‑150 (MQ=255) aaaGGCAGCAGCAACCAGCTGGCCAGTCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACatg > 2:199756/1‑150 (MQ=255) caACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGa > 2:299785/1‑150 (MQ=255) ccAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTa > 1:241999/1‑150 (MQ=255) ccAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCCGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTa < 2:381838/150‑1 (MQ=255) ccAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCCGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTa < 2:75006/150‑1 (MQ=255) cAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTAc < 2:74312/150‑1 (MQ=255) ccagATCATCGAGGACGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTACGTTGGCGCCaa < 1:281479/150‑1 (MQ=255) tGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTACGTTGGCGCCAACGGCACCTGGATGGGTAt < 1:294897/150‑1 (MQ=255) ttCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTACGTTGGCGCCAACGGCACCTGGATGGGTATCACCGc < 2:262274/150‑1 (MQ=255) aCATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCAGGCTGAACATGATGCCCAAGGAGTACGTTGGCGCCAACGGCACCTGGATGGGTATCACCGCCCGTACGCGCa > 1:64551/1‑150 (MQ=255) atcTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACatgatg < 2:137019/87‑1 (MQ=255) atcTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACatgatg > 1:137019/1‑87 (MQ=255) acCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTACGtt > 2:206302/1‑96 (MQ=255) acCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTACGtt < 1:206302/96‑1 (MQ=255) | TGGCGAAGCCATTGCCAAGGCCTATGAGGCCAAGACCGGCATTCACATCGACATCCGCAAAGGCAGCAGCAACCAGCTGGCCAGCCAGATCATCGAGGAAGGTGATCGTTCGCCGGCCGACATCATCTACACCGAAGAGTCCCCGCCCCTGAACAACCTGGGCGAGCTGGGCCTGCTGGCCAAGATTGACGACGCCACGCTGAACATGATGCCCAAGGAGTACGTTGGCGCCAACGGCACCTGGATGGGTATCACCGCCCGTACGCGCA > NC_002947/5549938‑5550206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |