| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,631,134 | A→G | 10.5% | N205D (AAC→GAC) | putA → | proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,631,134 | 0 | A | G | 10.5% | 98.9 / 3.4 | 38 | N205D (AAC→GAC) | putA | proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (16/18); new base G (2/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01 | |||||||||||
AGGAAGGCGTGGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGG > NC_002947/5630994‑5631281 | aGGAAGGCGTGGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCGACGCCGCCa > 1:416263/1‑150 (MQ=255) ggCGTGGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCGACGCCGCCACCTgg < 2:416263/150‑1 (MQ=255) ggCGTGGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTgg < 2:481829/150‑1 (MQ=255) cgcTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGgctgc < 2:362040/150‑1 (MQ=255) gCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCg > 2:534531/1‑150 (MQ=255) ccTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCgg > 1:574803/1‑150 (MQ=255) cGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCt > 2:3740/1‑150 (MQ=255) gCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTgg > 1:354390/1‑123 (MQ=255) gCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGCGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTgg < 2:354390/123‑1 (MQ=255) ccTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAg < 1:544992/150‑1 (MQ=255) tgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCAccc < 1:576441/150‑1 (MQ=255) gTATCCCCTACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATa > 2:468689/1‑150 (MQ=255) tATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATaa < 1:534531/150‑1 (MQ=255) aTCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAAc < 2:59341/150‑1 (MQ=255) cccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGaa > 1:489765/1‑150 (MQ=255) cGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAAc > 1:89610/1‑150 (MQ=255) aaGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGcc > 1:451710/1‑150 (MQ=255) aaGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGcc < 1:209903/150‑1 (MQ=255) tGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACctcctc < 2:451710/150‑1 (MQ=255) cAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCGACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCgg > 1:306652/1‑150 (MQ=255) gATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAAc > 2:175847/1‑109 (MQ=255) gATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAAc < 1:175847/109‑1 (MQ=255) tACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAgcgg > 2:419948/1‑150 (MQ=255) tACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAgcgg > 1:24481/1‑150 (MQ=255) aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCga < 2:24481/150‑1 (MQ=255) aGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCATGCTGGTCAGCACCCATAACGAAACCGGCATCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATcc < 2:89610/150‑1 (MQ=255) gCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCg > 2:544361/1‑150 (MQ=255) ccACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCaa < 2:451184/149‑1 (MQ=255) cggggCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCaa < 1:136512/120‑1 (MQ=255) cTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCaa > 2:136512/1‑122 (MQ=255) ggCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTc < 1:468689/150‑1 (MQ=255) aaCAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGAc > 2:274058/1‑150 (MQ=255) aGCCCGTCACTGTTCGTCGACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATg < 2:306652/150‑1 (MQ=255) aGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCgatga > 2:474451/1‑130 (MQ=255) aGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCgatga < 1:474451/130‑1 (MQ=255) ccGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGcc < 2:489765/150‑1 (MQ=255) tCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGa < 2:574803/150‑1 (MQ=255) tCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATgg > 1:234119/1‑150 (MQ=255) | AGGAAGGCGTGGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGG > NC_002947/5630994‑5631281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |