| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,813,279 | C→G | 17.4% | A48A (GCC→GCG) | argS → | arginine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,813,279 | 0 | C | G | 17.4% | 51.5 / 6.6 | 23 | A48A (GCC→GCG) | argS | arginine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base C (10/9); new base G (2/2); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
CCATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCA > NC_002947/5813134‑5813427 | ccATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCatga > 2:309202/1‑150 (MQ=255) aaaGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTg < 2:250956/150‑1 (MQ=255) gACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCatga > 1:201761/1‑142 (MQ=255) gACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCatga < 2:201761/142‑1 (MQ=255) aGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCa < 1:105751/150‑1 (MQ=255) ccTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCTAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCt > 1:503358/1‑150 (MQ=255) ccTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCATCATGGCGATGTTGCTg < 1:487076/118‑1 (MQ=255) ccTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCATCATGGCGATGTTGCTg > 2:487076/1‑118 (MQ=255) tCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCg > 1:51049/1‑150 (MQ=255) cACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGc < 1:407799/150‑1 (MQ=255) aCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGGTGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGcc > 1:436607/1‑150 (MQ=255) tCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGa < 2:51049/150‑1 (MQ=255) gAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGa < 2:341476/130‑1 (MQ=255) gAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGa > 1:341476/1‑130 (MQ=255) aGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCgaga > 2:550394/1‑150 (MQ=255) aCAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTcc < 1:133814/150‑1 (MQ=255) aGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCa > 2:368100/1‑150 (MQ=255) cGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCa < 2:503358/150‑1 (MQ=255) ccAGCATCATGGCGATGTTGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCa > 2:61146/1‑116 (MQ=255) ccAGCATCATGGCGATGTTGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCa < 1:61146/116‑1 (MQ=255) ccAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCg > 1:392234/1‑150 (MQ=255) gCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCACCGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACg > 1:477860/1‑150 (MQ=255) aTCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTg < 1:309202/150‑1 (MQ=255) ccATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCa > 1:153991/1‑150 (MQ=255) cATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtct > 1:605747/1‑125 (MQ=255) cATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtct < 2:605747/125‑1 (MQ=255) | CCATGAAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCA > NC_002947/5813134‑5813427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |