Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,827,811	G→C	14.8%	G223A (GGG→GCG) ‡	mtgA →	monofunctional biosynthetic peptidoglycan transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,827,811	0	G	C	14.8%	69.2 / 6.3	27	G223A (GGG→GCG) ‡	mtgA	monofunctional biosynthetic peptidoglycan transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (16/7);  new base C (2/2);  total (18/9)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGG  >  NC_002947/5827667‑5827960                                                                                                                                                 |                                                                                                                                                      tCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCa                                                                                                                                                  <  1:343031/150‑1 (MQ=255)           ccGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGAt                                                                                                                                        <  2:155146/150‑1 (MQ=255)                 cAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCGCCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCAGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCt                                                                                                                                  >  1:449875/1‑150 (MQ=255)                               cagcgcagcTGGCAGCGGTGCTGCCAAGCCCACTGGATGGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGACGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGaa                                                                                                                    >  1:356207/1‑150 (MQ=255)                                                    tgcCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAggcccggc                                                                                               >  1:50346/1‑150 (MQ=255)                                                        aaGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCtt                                                                                           >  2:360911/1‑150 (MQ=255)                                                          gCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTc                                                                                         >  2:366512/1‑150 (MQ=255)                                                                    gTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGACCCTTTCGCGGGTAAAc                                                                               >  1:309624/1‑150 (MQ=255)                                                                     tGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAAt                                                                                                          <  2:483757/122‑1 (MQ=255)                                                                     tGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAAt                                                                                                          >  1:483757/1‑122 (MQ=255)                                                                             cAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTa                                                                      >  1:393850/1‑150 (MQ=255)                                                                                  gaccgaGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGCTGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGc                                                                 >  2:86802/1‑150 (MQ=255)                                                                                  gaccgaGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCtt                                                                                                               <  1:102870/104‑1 (MQ=255)                                                                                  gaccgaGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCtt                                                                                                               >  2:102870/1‑104 (MQ=255)                                                                                     cgaGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCACTCCTACAAGCaaa                                                              >  2:401062/1‑149 (MQ=255)                                                                                                 tGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGc                                                  <  2:302721/150‑1 (MQ=255)                                                                                                   gCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGcct                                                >  1:513219/1‑150 (MQ=255)                                                                                                         cgggccggCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTcgc                                          >  1:158593/1‑150 (MQ=255)                                                                                                                gCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCaaa                                   >  1:236491/1‑150 (MQ=255)                                                                                                                   ggATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAAccc                                >  2:154542/1‑150 (MQ=255)                                                                                                                       tCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCgcgc                            <  2:236491/150‑1 (MQ=255)                                                                                                                                 atcaggtaGCTGGGCCCGCCCAGCTGCCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCaa                  >  1:201147/8‑150 (MQ=38)                                                                                                                                  tcaggtaGCTGGGCCCGCCCAGCTGGCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCcgc                              <  1:187486/131‑1 (MQ=38)                                                                                                                                  tcaggtaGCTGGGCCCGCCCAGCTGGCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCcgc                              >  2:187486/7‑137 (MQ=38)                                                                                                                                  tcaggtaGCTGGGCCCGCCCAGCTGCCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAAc                 <  2:201147/144‑1 (MQ=39)                                                                                                                                   gAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAAcc                <  1:154542/150‑1 (MQ=255)                                                                                                                                           tGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGGAAACCCGCGCCTACAGGCAACCGCATcccc        >  1:120513/1‑150 (MQ=255)                                                                                                                                               cgggcCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACgg                                                                   >  2:46821/1‑87 (MQ=255)                                                                                                                                               cgggcCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACgg                                                                   <  1:46821/87‑1 (MQ=255)                                                                                                                                                 ggcCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAgg  >  1:229838/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                      TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGCCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGG  >  NC_002947/5827667‑5827960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑